Publication Date:
1996-03-08
Description:
Transcriptional regulatory elements have been shown to be necessary but not sufficient for the developmental regulation of immunoglobulin gene rearrangement in mouse precursor B cells. In the chicken lambda light chain locus, additional elements in the V-J intervening sequence are involved in negative and positive regulation of rearrangement. Here, mutation of the mouse homolog of a chicken element, located in the V(K)-J(K) intervening sequence upstream of the J(K) cluster, was shown to significantly decrease rearrangement. This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ferradini, L -- Gu, H -- De Smet, A -- Rajewsky, K -- Reynaud, C A -- Weill, J C -- New York, N.Y. -- Science. 1996 Mar 8;271(5254):1416-20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉INSERM U373, Institut Necker, Faculte de Medecine, Universite Paris V, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8596914" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Animals
;
B-Lymphocytes/cytology/immunology
;
Base Sequence
;
Chimera
;
Enhancer Elements, Genetic
;
*Gene Rearrangement, B-Lymphocyte
;
Gene Rearrangement, T-Lymphocyte
;
Gene Targeting
;
*Genes, Immunoglobulin
;
Immunoglobulin J-Chains/*genetics
;
Immunoglobulin Variable Region/genetics
;
Immunoglobulin kappa-Chains/*genetics
;
Introns
;
Mice
;
Mice, Inbred C57BL
;
Mice, SCID
;
Molecular Sequence Data
;
Mutation
;
Recombination, Genetic
;
*Regulatory Sequences, Nucleic Acid
;
Stem Cells
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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