Publication Date:
2012-10-23
Description:
During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to form an RNAP-promoter open complex. We have determined crystal structures at 2.9 and 3.0 A resolution of functional transcription initiation complexes comprising Thermus thermophilus RNA polymerase, sigma(A), and a promoter DNA fragment corresponding to the transcription bubble and downstream double-stranded DNA of the RNAP-promoter open complex. The structures show that sigma recognizes the -10 element and discriminator element through interactions that include the unstacking and insertion into pockets of three DNA bases and that RNAP recognizes the -4/+2 region through interactions that include the unstacking and insertion into a pocket of the +2 base. The structures further show that interactions between sigma and template-strand single-stranded DNA (ssDNA) preorganize template-strand ssDNA to engage the RNAP active center.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593053/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593053/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Yu -- Feng, Yu -- Chatterjee, Sujoy -- Tuske, Steve -- Ho, Mary X -- Arnold, Eddy -- Ebright, Richard H -- AI072766/AI/NIAID NIH HHS/ -- GM41376/GM/NIGMS NIH HHS/ -- P30 EB009998/EB/NIBIB NIH HHS/ -- R01 AI072766/AI/NIAID NIH HHS/ -- R01 GM041376/GM/NIGMS NIH HHS/ -- R37 GM041376/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2012 Nov 23;338(6110):1076-80. doi: 10.1126/science.1227786. Epub 2012 Oct 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Waksman Institute, and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23086998" target="_blank"〉PubMed〈/a〉
Keywords:
Crystallography, X-Ray
;
DNA, Single-Stranded/chemistry
;
DNA-Directed RNA Polymerases/*chemistry
;
*Gene Expression Regulation, Bacterial
;
Promoter Regions, Genetic
;
Protein Conformation
;
Sigma Factor/*chemistry
;
Thermus thermophilus/*enzymology/*genetics
;
*Transcription Initiation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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