Publication Date:
2011-11-15
Description:
In the mammalian genome, 5'-CpG-3' dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by using genomic-scale reduced representation bisulfite sequencing (RRBS). Demethylation at the erythroid-specific beta-globin locus was coincident with global DNA demethylation at most genomic elements. Global demethylation was continuous throughout differentiation and required rapid DNA replication. Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230325/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230325/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shearstone, Jeffrey R -- Pop, Ramona -- Bock, Christoph -- Boyle, Patrick -- Meissner, Alexander -- Socolovsky, Merav -- DK32520/DK/NIDDK NIH HHS/ -- R01 HL084168/HL/NHLBI NIH HHS/ -- R01 HL084168-02/HL/NHLBI NIH HHS/ -- R01 HL084168-03/HL/NHLBI NIH HHS/ -- R01 HL084168-04/HL/NHLBI NIH HHS/ -- R01 HL084168-04S1/HL/NHLBI NIH HHS/ -- R01 HL084168-05/HL/NHLBI NIH HHS/ -- T32-130807/PHS HHS/ -- New York, N.Y. -- Science. 2011 Nov 11;334(6057):799-802. doi: 10.1126/science.1207306.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01605, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22076376" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CpG Islands
;
*DNA Methylation
;
DNA Replication
;
Dinucleoside Phosphates/metabolism
;
Embryo, Mammalian
;
Erythroblasts/*metabolism
;
*Erythropoiesis
;
Gene Expression Regulation, Developmental
;
Genome
;
Liver/embryology
;
Locus Control Region
;
Long Interspersed Nucleotide Elements
;
Mice
;
S Phase
;
Sequence Analysis, DNA
;
Transcription, Genetic
;
beta-Globins/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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