Publication Date:
1988-02-05
Description:
An expression vector for the epidermal growth factor (EGF) receptor was introduced into the 32D myeloid cell line, which is devoid of EGF receptors and absolutely dependent on interleukin-3 (IL-3) for its proliferation and survival. Expression of the EGF receptor conferred the ability to utilize EGF for transduction of a mitogenic signal. When the transfected cells were propagated in EGF, they exhibited a more mature myeloid phenotype than was observed under conditions of IL-3-directed growth. Moreover, exposure to EGF led to a rapid stimulation of phosphoinositide metabolism, while IL-3 had no detectable effect on phosphoinositide turnover either in control or EGF receptor-transfected 32D cells. Although the transfected cells exhibited high levels of functional EGF receptors, they remained nontumorigenic. In contrast, transfection of v-erbB, an amino-terminal truncated form of the EGF receptor with constitutive tyrosine kinase activity, not only abrogated the IL-3 growth factor requirement of 32D cells, but caused them to become tumorigenic in nude mice. These results show that a naive hematopoietic cell expresses all of the intracellular components of the EGF-signaling pathway necessary to evoke a mitogenic response and sustain continuous proliferation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pierce, J H -- Ruggiero, M -- Fleming, T P -- Di Fiore, P P -- Greenberger, J S -- Varticovski, L -- Schlessinger, J -- Rovera, G -- Aaronson, S A -- New York, N.Y. -- Science. 1988 Feb 5;239(4840):628-31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3257584" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Division
;
Cell Line
;
*Cloning, Molecular
;
DNA Replication/drug effects
;
Epidermal Growth Factor/metabolism/pharmacology
;
Genetic Vectors
;
Hematopoietic Stem Cells/cytology/drug effects/*metabolism
;
Interleukin-3/*pharmacology
;
Receptor, Epidermal Growth Factor/*genetics/metabolism
;
*Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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