Publication Date:
2002-08-10
Description:
In Saccharomyces cerevisiae, the telomerase components Est2p, TLC1 RNA, Est1p, and Est3p are thought to form a complex that acts late during chromosome replication (S phase) upon recruitment by Cdc13p, a telomeric DNA binding protein. Consistent with this model, we show that Est1p, Est2p, and Cdc13p are telomere-associated at this time. However, Est2p, but not Est1p, also binds telomeres before late S phase. The cdc13-2 allele has been proposed to be defective in recruitment, yet Est1p and Est2p telomere association persists in cdc13-2 cells. These findings suggest a model in which Est1p binds telomeres late in S phase and interacts with Cdc13p to convert inactive, telomere-bound Est2p to an active form.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Taggart, Andrew K P -- Teng, Shu-Chun -- Zakian, Virginia A -- GM43265/GM/NIGMS NIH HHS/ -- T32 CA09528-16/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 9;297(5583):1023-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12169735" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
*Cell Cycle
;
DNA, Fungal/genetics/metabolism
;
DNA-Binding Proteins/genetics/metabolism
;
Enzyme Activation
;
G1 Phase
;
Genes, Fungal
;
Models, Biological
;
Polymerase Chain Reaction
;
Precipitin Tests
;
RNA, Fungal/genetics/metabolism
;
S Phase
;
Saccharomyces cerevisiae/cytology/enzymology/genetics/*metabolism
;
Saccharomyces cerevisiae Proteins/genetics/*metabolism
;
Telomerase/genetics/*metabolism
;
Telomere/*metabolism
;
*Telomere-Binding Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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