Publication Date:
2002-06-08
Description:
Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bousso, Philippe -- Bhakta, Nirav R -- Lewis, Richard S -- Robey, Ellen -- AI32985/AI/NIAID NIH HHS/ -- AI42033/AI/NIAID NIH HHS/ -- GM45374/GM/NIGMS NIH HHS/ -- R01 GM045374/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2002 Jun 7;296(5574):1876-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12052962" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigen Presentation
;
Benzopyrans
;
CD8-Positive T-Lymphocytes/immunology
;
Cell Aggregation
;
*Cell Communication
;
Cell Movement
;
Cell Size
;
Coculture Techniques
;
Fluoresceins
;
Fluorescent Dyes
;
Histocompatibility Antigens Class I/*immunology
;
Lasers
;
Mice
;
Mice, Inbred C57BL
;
Mice, Transgenic
;
Microscopy/methods
;
Naphthols
;
Organ Culture Techniques
;
Photons
;
Receptors, Antigen, T-Cell/*immunology
;
Rhodamines
;
Signal Transduction
;
Stromal Cells/immunology/*physiology
;
Succinimides
;
T-Lymphocyte Subsets/immunology
;
T-Lymphocytes/cytology/immunology/*physiology
;
Thymus Gland/cytology/*immunology
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
