Publication Date:
2013-12-03
Description:
The increasing demands placed on natural resources for fuel and food production require that we explore the use of efficient, sustainable feedstocks such as brown macroalgae. The full potential of brown macroalgae as feedstocks for commercial-scale fuel ethanol production, however, requires extensive re-engineering of the alginate and mannitol catabolic pathways in the standard industrial microbe Saccharomyces cerevisiae. Here we present the discovery of an alginate monomer (4-deoxy-L-erythro-5-hexoseulose uronate, or DEHU) transporter from the alginolytic eukaryote Asteromyces cruciatus. The genomic integration and overexpression of the gene encoding this transporter, together with the necessary bacterial alginate and deregulated native mannitol catabolism genes, conferred the ability of an S. cerevisiae strain to efficiently metabolize DEHU and mannitol. When this platform was further adapted to grow on mannitol and DEHU under anaerobic conditions, it was capable of ethanol fermentation from mannitol and DEHU, achieving titres of 4.6% (v/v) (36.2 g l(-1)) and yields up to 83% of the maximum theoretical yield from consumed sugars. These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enquist-Newman, Maria -- Faust, Ann Marie E -- Bravo, Daniel D -- Santos, Christine Nicole S -- Raisner, Ryan M -- Hanel, Arthur -- Sarvabhowman, Preethi -- Le, Chi -- Regitsky, Drew D -- Cooper, Susan R -- Peereboom, Lars -- Clark, Alana -- Martinez, Yessica -- Goldsmith, Joshua -- Cho, Min Y -- Donohoue, Paul D -- Luo, Lily -- Lamberson, Brigit -- Tamrakar, Pramila -- Kim, Edward J -- Villari, Jeffrey L -- Gill, Avinash -- Tripathi, Shital A -- Karamchedu, Padma -- Paredes, Carlos J -- Rajgarhia, Vineet -- Kotlar, Hans Kristian -- Bailey, Richard B -- Miller, Dennis J -- Ohler, Nicholas L -- Swimmer, Candace -- Yoshikuni, Yasuo -- England -- Nature. 2014 Jan 9;505(7482):239-43. doi: 10.1038/nature12771. Epub 2013 Dec 1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA [2]. ; 1] Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA [2] Manus Biosynthesis Inc., 790 Memorial Drive, Suite 102, Cambridge, Massachusetts 02139 (C.N.S.S.); Calysta Energy, 1140 O'Brien Drive, Menlo Park, California 94025 (D.D.R.); Sutro Biopharma lnc., 310 Utah Avenue, Suite 150, South San Francisco, California 94080, USA (A.G.); Total New Energies USA, 5858 Horton Street, Emeryville, California 94560 (S.A.T.; V.R.). ; Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA. ; Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, Michigan 48824-1226, USA. ; Statoil ASA, Statoil Research Centre, Arkitekt Ebbells vei 10, Rotvoll, 7005 Trondheim, Norway. ; 1] Bio Architecture Lab Inc., 604 Bancroft Way, Suite A, Berkeley, California 94710, USA [2] BALChile S.A., Badajoz 100, Oficina 1404, Las Condes, Santiago 7550000, Chile [3] BAL Biofuels S.A., Badajoz 100, Oficina 1404, Las Condes, Santiago 7550000, Chile.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24291791" target="_blank"〉PubMed〈/a〉
Keywords:
Alginates/metabolism
;
Anaerobiosis
;
Ascomycota/genetics/metabolism
;
Biofuels/*supply & distribution
;
Biotechnology
;
*Carbohydrate Metabolism
;
Carrier Proteins/genetics/metabolism
;
Ethanol/*metabolism
;
Evolution, Molecular
;
Fermentation
;
Genetic Complementation Test
;
*Genetic Engineering
;
Glucuronic Acid/metabolism
;
Hexuronic Acids/metabolism
;
Mannitol/metabolism
;
Phaeophyta/genetics/*metabolism
;
Quinic Acid/metabolism
;
Reproducibility of Results
;
Saccharomyces cerevisiae/genetics/*metabolism
;
Seaweed/genetics/metabolism
;
Uronic Acids/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
