Publication Date:
2012-09-18
Description:
Unidirectional fluid flow plays an essential role in the breaking of left-right (L-R) symmetry in mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We report that the Ca(2+) channel Polycystin-2 (Pkd2) is required specifically in the perinodal crown cells for sensing the nodal flow. Examination of mutant forms of Pkd2 shows that the ciliary localization of Pkd2 is essential for correct L-R patterning. Whereas Kif3a mutant embryos, which lack all cilia, failed to respond to an artificial flow, restoration of primary cilia in crown cells rescued the response to the flow. Our results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711115/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711115/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoshiba, Satoko -- Shiratori, Hidetaka -- Kuo, Ivana Y -- Kawasumi, Aiko -- Shinohara, Kyosuke -- Nonaka, Shigenori -- Asai, Yasuko -- Sasaki, Genta -- Belo, Jose Antonio -- Sasaki, Hiroshi -- Nakai, Junichi -- Dworniczak, Bernd -- Ehrlich, Barbara E -- Pennekamp, Petra -- Hamada, Hiroshi -- P30 DK090744/DK/NIDDK NIH HHS/ -- P50 DK057328/DK/NIDDK NIH HHS/ -- R01 DK087844/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2012 Oct 12;338(6104):226-31. doi: 10.1126/science.1222538. Epub 2012 Sep 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, 565-0871 Osaka, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22983710" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Body Fluids/physiology
;
*Body Patterning
;
Calcium/metabolism
;
Cilia/metabolism/physiology
;
Embryo, Mammalian/anatomy & histology/cytology/*physiology
;
Gene Expression Regulation, Developmental
;
Intercellular Signaling Peptides and Proteins/metabolism
;
Kinesin/genetics
;
Left-Right Determination Factors/genetics/*metabolism
;
Mice
;
Mice, Mutant Strains
;
Mutation
;
Organizers, Embryonic/cytology/*physiology
;
Signal Transduction
;
TRPP Cation Channels/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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