Publikationsdatum:
2006-03-18
Beschreibung:
The parietal-eye photoreceptor is unique because it has two antagonistic light signaling pathways in the same cell-a hyperpolarizing pathway maximally sensitive to blue light and a depolarizing pathway maximally sensitive to green light. Here, we report the molecular components of these two pathways. We found two opsins in the same cell: the blue-sensitive pinopsin and a previously unidentified green-sensitive opsin, which we name parietopsin. Signaling components included gustducin-alpha and Galphao, but not rod or cone transducin-alpha. Single-cell recordings demonstrated that Go mediates the depolarizing response. Gustducin-alpha resembles transducin-alpha functionally and likely mediates the hyperpolarizing response. The parietopsin-Go signaling pair provides clues about how rod and cone phototransduction might have evolved.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Su, Chih-Ying -- Luo, Dong-Gen -- Terakita, Akihisa -- Shichida, Yoshinori -- Liao, Hsi-Wen -- Kazmi, Manija A -- Sakmar, Thomas P -- Yau, King-Wai -- EY06837/EY/NEI NIH HHS/ -- R01 DC006904/DC/NIDCD NIH HHS/ -- R01 DC006904-01/DC/NIDCD NIH HHS/ -- R01 DC006904-02/DC/NIDCD NIH HHS/ -- R01 EY006837/EY/NEI NIH HHS/ -- R01 EY006837-16A1/EY/NEI NIH HHS/ -- R01 EY006837-17/EY/NEI NIH HHS/ -- R01 EY006837-18/EY/NEI NIH HHS/ -- R01 EY006837-19/EY/NEI NIH HHS/ -- R01 EY014596/EY/NEI NIH HHS/ -- R01 EY014596-01/EY/NEI NIH HHS/ -- R01 EY014596-02/EY/NEI NIH HHS/ -- R01 EY014596-03/EY/NEI NIH HHS/ -- R01 EY014596-04/EY/NEI NIH HHS/ -- R37 EY006837/EY/NEI NIH HHS/ -- R37 EY006837-15S1/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 2006 Mar 17;311(5767):1617-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. chih-ying.su@yale.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16543463" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
3',5'-Cyclic-GMP Phosphodiesterases/metabolism
;
Amino Acid Sequence
;
Animals
;
*Biological Evolution
;
Cell Line
;
Cyclic GMP/metabolism
;
GTP-Binding Protein alpha Subunits/genetics/physiology
;
Humans
;
Lizards/genetics/*physiology
;
Molecular Sequence Data
;
*Ocular Physiological Phenomena
;
Patch-Clamp Techniques
;
Photoreceptor Cells, Vertebrate/chemistry/*physiology
;
Rod Opsins/analysis/genetics/*physiology
;
Transducin/genetics/physiology
;
*Vision, Ocular
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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