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  • Argentina  (3)
  • Cell & Developmental Biology  (3)
  • *Biological Evolution  (2)
  • 1
    ISSN: 0886-1544
    Keywords: lipocytes ; liver cirrhosis ; myofibroblasts ; myosin gene ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fat-storing cells (FSC, lipocytes, or Ito cells) of liver store vitamin A and are the main producers of extracellular matrix in normal and cirrhotic liver. During liver injury, FSC undergo an activation process characterized by a decrease in vitamin A storage and an increase in cell proliferation and extracellular matrix deposition. This activation process also occurs upon culturing FSC from normal liver. In contrast to most cells of nonmuscle origin, activated FSC express two cytoskeletal proteins normally found in muscle, desmin, and smooth muscle α-actin. Based on their strategic perisinusoidal location, it has been hypothesized that FSC play a role in regulating blood flow. However, the nature of the contractile elements involved in this process remains to be determined. In this communication we demonstrate the presence of a sarcomeric myosin in proteins solubilized from liver biomatrix. In addition we demonstrate the expression of sarcomeric myosin heavy chain (MHC) mRNA and protein in two FSC clones derived from a CCl4-cirrhotic rat liver (CFSC). Through cloning the cDNA corresponding to the MHC gene expressed in these cells we demonstrate that it encodes fast IId skeletal MHC and thus represents a marker normally seen in adult muscle. The unexpected expression of an adult stage skeletal muscle molecular motor in FSC from cirrhotic liver is consistent with the proposed specialized contractile capacity of these cells. © 1993 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 36 (1993), S. 23-32 
    ISSN: 1040-452X
    Keywords: Sperm ; Aster ; Bovine ; Centrosome ; Polyspermy ; Adrogenote ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Chromatin and microtubule configurations during the first cell cycle of bovine zygotes were analyzed by DNA staining and microtubule immunolocalization using an IVM/IVF system and oocytes matured and fertilized in vivo, in order to investigate the origin of the active centrosome and to characterize the nuclear and the cytoplasmic changes following bovine fertilization. Our results suggest that the paternal centrosome is active during early zygotic development, forming a conspicuous sperm aster soon after fertilization. We also report that polyspermy in bovine eggs, leads to the formation of numerous sperm asters with different degrees of association with the chromatin. The maternal structures in both monospermic and polyspermic zygotes can be lost or degenerate. Consequently, these cells may resume the first cell cycle as androgenotes, very often with several types of mitotic activity taking place in different regions of the cell cytoplasm at the same time. As indicated by a comparison of monospermic and polyspermic fertilization rates to rates of development, it is possible that some androgenetic embryos cleave and develop to the blastocyst stage. © 1993 Wiley-Liss, Inc.
    Additional Material: 1 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 34 (1993), S. 33-42 
    ISSN: 1040-452X
    Keywords: Spindle ; Oocyte ; Mammalian ; Centrosome ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Artificial activation and nuclear transfer in rabbit oocytes have been used in past years in an attempt to develop viable techniques for cloning in cattle. The procedures established in our laboratory, using the rabbit as a model, consistently lead to high rates of development to the blastocyst stage. However, the rate of embryos developing to term is considerably lower. In the present study, we undertook a detailed immunocytochemical study of the patterns of both microtubules and chromatin during the first cell cycle of electrical pulse-activated oocytes and of nuclear transfer embryos. Our goal was to investigate the responses of the cell to the different stimuli applied and to establish the sequence of events leading to first cleavage in the absence of normal fertilization. Our results show that, in both electrically activated oocytes and nuclear transfer embryos, although the initial development patterns are rather unusual, embryos become synchronized at the time of the formation of a pronuclear-like structure, and then organize metaphase spindles and cleave. These spindles consistently present small defects, suggesting that problems in the formation of the mitotic apparatus during the first cell cycle may have a long-term effect leading to embryo mortality. © 1993 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 4
    Publication Date: 2014-11-05
    Description: Potato cyst nematodes, G. rostochiensis and G. pallida, are the most economically important nematode pests of potatoes worldwide and are subject to strict quarantine regulations in many countries. Globodera ellingtonae was recently described from Oregon (USA), with its host-plant in the field being still unknown. Roots of Andean potatoes from the North of Argentina have been found attacked by this nematode, providing further evidence that this is a potato cyst nematode species, along with G. pallida and G. rostochiensis. New information about morphological, biological and molecular aspects of G. ellingtonae is provided for diagnostic purposes. The Argentine population showed morphological differences from specimens from Oregon; therefore, new diagnostic characters were defined to differentiate G. ellingtonae from its closest species. The Hsp90 gene was shown to be a good diagnostic marker for discriminating the three PCN species. The importance of the detection of G. ellingtonae on potatoes in the Andean region is not restricted to a regional level, since the nematode is also present in USA. This species can pose a serious problem to potato crop, especially when infected tubers are used as seeds. The distribution in the South American Andes is likely to extend the currently known distribution areas because cysts are passively transported. There is a need to evaluate the possible damage it may cause to potato crops. Morphological and molecular diagnoses conducted in this work provide fundamental information for the protection of potato crops not only in those countries in the Americas where the species has already been detected, but also worldwide.
    Keywords: Andean potato ; Argentina ; diagnosis ; Hsp90 gene ; morphology ; potato cyst nematode ; 42.76 ; 42.64
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/16697 | 9602 | 2016-05-04 14:28:48 | 16697 | Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata, Argentina
    Publication Date: 2021-07-08
    Description: La glaciaciones ocurridas en la región expusieron en diferente grado la actual plataforma continental Argentina (océano Atlántico sudoccidental). Este trabajo de divulgación científica da a conocer un modelo de evolución paleogeográfica de las costas de la Pampa y la Patagonia, y brinda la posibilidad de localizar temporalmente la formación de los principales rasgos costeros del extremo sur de América del sur. Se incluyen además otras lecturas sugeridas relacionadas al tema.
    Description: Reprinted as: pp.11-17 in, Ciencia del Mar. Volumen temático 1, 12/2014; Asociación Ciencia Hoy. ISBN: 978-987-45584-0-4 (Special issue dedicated to 'Marine Sciences: 1988-2014')
    Keywords: Biology ; Ecology ; Oceanography ; PSW ; Argentina ; marine environment ; Continental shelves ; shelf geology ; glaciation ; palaeogeography ; sea level changes ; models
    Repository Name: AquaDocs
    Type: article
    Format: application/pdf
    Format: application/pdf
    Format: 50-56
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  • 6
    Publication Date: 2024-01-12
    Description: Potato cyst nematodes, G. rostochiensis and G. pallida, are the most economically important nematode pests of potatoes worldwide and are subject to strict quarantine regulations in many countries. Globodera ellingtonae was recently described from Oregon (USA), with its host-plant in the field being still unknown. Roots of Andean potatoes from the North of Argentina have been found attacked by this nematode, providing further evidence that this is a potato cyst nematode species, along with G. pallida and G. rostochiensis. New information about morphological, biological and molecular aspects of G. ellingtonae is provided for diagnostic purposes. The Argentine population showed morphological differences from specimens from Oregon; therefore, new diagnostic characters were defined to differentiate G. ellingtonae from its closest species. The Hsp90 gene was shown to be a good diagnostic marker for discriminating the three PCN species. The importance of the detection of G. ellingtonae on potatoes in the Andean region is not restricted to a regional level, since the nematode is also present in USA. This species can pose a serious problem to potato crop, especially when infected tubers are used as seeds. The distribution in the South American Andes is likely to extend the currently known distribution areas because cysts are passively transported. There is a need to evaluate the possible damage it may cause to potato crops. Morphological and molecular diagnoses conducted in this work provide fundamental information for the protection of potato crops not only in those countries in the Americas where the species has already been detected, but also worldwide.
    Keywords: Andean potato ; Argentina ; diagnosis ; Hsp90 gene ; morphology ; potato cyst nematode
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 7
    Publication Date: 2013-10-19
    Description: The site of Dmanisi, Georgia, has yielded an impressive sample of hominid cranial and postcranial remains, documenting the presence of Homo outside Africa around 1.8 million years ago. Here we report on a new cranium from Dmanisi (D4500) that, together with its mandible (D2600), represents the world's first completely preserved adult hominid skull from the early Pleistocene. D4500/D2600 combines a small braincase (546 cubic centimeters) with a large prognathic face and exhibits close morphological affinities with the earliest known Homo fossils from Africa. The Dmanisi sample, which now comprises five crania, provides direct evidence for wide morphological variation within and among early Homo paleodemes. This implies the existence of a single evolving lineage of early Homo, with phylogeographic continuity across continents.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lordkipanidze, David -- Ponce de Leon, Marcia S -- Margvelashvili, Ann -- Rak, Yoel -- Rightmire, G Philip -- Vekua, Abesalom -- Zollikofer, Christoph P E -- New York, N.Y. -- Science. 2013 Oct 18;342(6156):326-31. doi: 10.1126/science.1238484.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Georgian National Museum, 3 Purtseladze Street, 0105 Tbilisi, Georgia.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24136960" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biological Evolution ; Brain/anatomy & histology ; Dentition ; Face/*anatomy & histology ; *Fossils ; Georgia (Republic) ; Hominidae/*anatomy & histology/growth & development ; Humans ; Organ Size ; Phylogeography ; Skull/*anatomy & histology/growth & development ; Skull Base/anatomy & histology/growth & development ; Temporal Bone/anatomy & histology/growth & development
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Publication Date: 2014-04-26
    Description: Schwartz et al. hold that variation among the Dmanisi skulls reflects taxic diversity. The morphological observations to support their hypothesis, however, are partly incorrect, and not calibrated against intraspecific variation in living taxa. After proper adjustment, Schwartz et al.'s data are fully compatible with the hypothesis of a single paleodeme of early Homo at Dmanisi.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zollikofer, Christoph P E -- Ponce de Leon, Marcia S -- Margvelashvili, Ann -- Rightmire, G Philip -- Lordkipanidze, David -- New York, N.Y. -- Science. 2014 Apr 25;344(6182):360. doi: 10.1126/science.1250081.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Anthropological Institute and Museum, University of Zurich, Zurich, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24763573" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biological Evolution ; Face/*anatomy & histology ; *Fossils ; Hominidae/*anatomy & histology ; Humans ; Skull/*anatomy & histology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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