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  • Biochemistry and Biotechnology  (3)
  • (Mono)terpene  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Trees 1 (1987), S. 88-93 
    ISSN: 1432-2285
    Keywords: 1Gas chromatography ; Picea abies ; Solvent extraction ; (Mono)terpene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An analysis was made of the effects of different sampling and extraction techniques on the amounts and pattern of monoterpenoids isolated from needles of Norway spruce. The following isolation and analysis procedure was finally adopted: liquid nitrogen-cooled needles were pulverized by a microdismembrator, extracted with pentane overnight at 2°–3°C and concentrated to a volume not less than 3 ml/g fresh weight on a Vigreux column. The crude extract was injected splitless (with solvent split) onto a cold programmed temperature vaporized (PTV) precolumn of a gas chromatograph and the vaporizable compounds heated to a capillary column. This method was tested for production of artefacts and quantitative extraction and applied to needles of eleven 80-year-old spruce trees.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2285
    Keywords: Bavaria ; Genetic variation ; Needle ; Picea abies ; (Mono)terpene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary As genetic diversity is an important cause of variation in needle monoterpenes of conifers, the tree-to-tree variation was studied for Picea abies (L.) Karst. at eight different locations in three mountainous regions of southern Germany (Bavaria). The tree-to-tree variation, expressed by the coefficients of variation, is high, ranging between 50% and 70%. The maximum concentrations per tree are up to 10 times higher than the minimum ones. The trees from all locations show similar patterns of variation, which is significant when investigating the influence of external parameters like air pollution or infestation by pathogens. By means of cluster analysis five types of terpene patterns were clearly revealed. Two main groups were distinguished by their proportion of myrcene, and they are further divided into subgroups. The patterns are stable throughout different needle ages and dates of sampling. Apart from one location, there is no obvious geographical arrangement of the five patterns. The fact that no relationship exists between the genotypical classification and the total concentrations agrees well with the hypothesis that most of the variability based on the relative amounts is due to genetic variation, whereas the variation based on the absolute amounts reflects environmental factors.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 765-767 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 507-525 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxygen transfer measurements using a dynamic method and evaluated with an appropriate mathematical model have been made on a tubular loop bioreactor. Correlations of the type used in tank systems are used to describe the influence of power and aeration rate on the mass transfer coefficient. Yeast cultures grown on hydrocarbon and glucose substrates show growth characteristics similar to conventional tank results. Model considerations for large-scale tubular fermentors allow for the prediction of the steady-state oxygen profiles and maximum reactor length. Combination with two-phase flow and oxygen transfer correlations yields a design procedure for commercial scale tubular loop fermentors.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 1613-1635 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 22 m long. 20 liter tubular loop fermentor (TLF) has been tested for oxygen transfer characteristics and as a reactor for mycelial growth. Model calculations show that the flow pressure drop has an important influence on the axial oxygen profiles. A design model that accounts for this influence is presented. Using the model, KLa values are calculated from the results of sulfite oxidation experiments. These are correlated with power consumption and aeration rates. The KLa dependence on aeration rate was found to be less than found with tank reactors. The growth kinetics of three metabolite-producing mycelial organisms in the TLF are presented: a Streptomyces, a Fusarium, and a Acrophialophora. In order to determine the influence of reactor type on the growth and product formation, these cultures have been grown in tanks and shake flasks. The antibiotic, product spectrum of Streptomyces is compared on the basis of inhibition tests and it is shown that the distribution of products is reactor dependent. The Fusarium culture produced a previously unknown metabolite, whose concentration in the loop fermentor was four times higher than in a shake flask. The Acrophialophora culture grew twice as fast in the loop fermentor, but produced essentially none of the specific product. Power Consumptions of up to 8 kW/m3 in the tubular fermentor did not appear to harm the mycelia.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
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