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  • (ELISA, monoclonal antibody)  (1)
  • 551.48  (1)
  • 1
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    Drought Characteristics Derived Based on the Standardized Streamflow Index: A Large Sample Comparison for Parametric and Nonparametric Methods (2021)
    Details
    Publikationsdatum: 2021-10-01
    Beschreibung: The streamflow drought hazard can be characterized in a variety of ways, including using different indices. Traditionally, percentile-based indices, such as Q95 (the flow exceeded 95% of time), have been used by the hydrological community. Recently, the use of anomaly indices such as the Standardized Streamflow Index (SSI), a probability index-based approach adopted from the climatological community, has increased in popularity. The SSI can be calculated based on various (non)parametric methods. Up to now, there is no consensus which method to use. This study aims to raise awareness how the inherent sensitivity of the SSI to the used method influences derived drought characteristics. We compared SSI time series computed with seven different probability distributions and two fitting methods as well as with different nonparametric methods for 369 rivers across Europe. Results showed that SSI time series and associated drought characteristics are indeed sensitive to the method of choice. A resampling experiment demonstrated the sensitivity of the parametric SSI to properties of both the low and high end of the sample. Such sensitivities might hinder a fair comparison of drought in space and time and highlight the need for a clear recommendation which method to use. We could recommend overall suitable methods, for example, from the parametric approaches, the Tweedie distribution has several advantageous properties such as a low rejection rate (2%) and a lower bound at zero. However, the most suitable method depends on the used evaluation criteria. Rather, we stress that shown approach-specific sensitivities and uncertainties should be carefully considered.
    Schlagwort(e): 551.48 ; drought ; parametric ; drought identification ; Standardized Streamflow Index ; sensitivity analyses ; nonparametric
    Sprache: Englisch
    Materialart: map
    Standort Signatur Erwartet Verfügbarkeit
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    CITATION GEO-LEO
  • 2
    Digitale Medien
    Digitale Medien
    Quantification of rapid, transient increases in jasmonic acid in wounded plants using a monoclonal antibody (1993)
    Springer
    Planta 191 (1993), S. 86-94 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Avena ; Bryonia ; Jasmonate analysis ; (ELISA, monoclonal antibody) ; Jasmonic acid (woundinduced) ; Methyljasmonate (tritium labeled) ; Wounding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A monoclonal antibody (MAB JAH1-8-B4) for the analysis of 3R, 7R-jasmonic acid and its methyl ester is described. An IgG1(kappa) immunoglobulin, MAB JAH1-8-B4, was used to set up a competitive enzymelinked immunoassay employing 3R, 7R-jasmonate coupled to alkaline phosphatase as tracer. The assay has a linearity range (logit/log) between 50 fmol and 50 pmol (approx. 10 pg-10 ng) of 3R, 7R-methyljasmonate, the assay standard. A procedure combining prepurification of plant extracts by solid-phase extraction, followed by high-performance liquid chromatography and quantitation has been worked out, which uses 4 g of fresh plant material and has a detection limit between 0.2 and 0.4 μg of 3R, 7R-jasmonic acid (determined as its methyl ester) per kg of tissue, depending on the tissue. Internal standards of 3R, 7R-methyljasmonate, added to split samples during extraction as well as a second internal standard, 3R, 7R-methyljasmonate-[O-C3H3], added to all samples prior to methylation, served to correct for workup losses and for the monitoring of Chromatographie separations. Using this assay, it was found that levels of jasmonic acid rise immediately and transiently in the tissues analyzed as a consequence of wounding. These data provide further and direct evidence for the hypothesis that wound-induction of the plant defense reactions is mediated by endogenous jasmonates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00240899
    Standort Signatur Erwartet Verfügbarkeit
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