ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Gas chromatography/isotope ratio mass spectrometry (GC/IRMS) allows the detection of low 13C enrichment. However, this method needs to set up new metabolite derivatives suitable for GC/IRMS. In this work we describe a new derivative for plasma lactate analysis. After plasma extraction, diazomethane is used to methylate the lactate carboxyl group. The lactate methylester reacts with acetic anhydride in pyridine to give an acetylated methyl ester of lactate. The isotopic 13C enrichment of this compound is determined by GC/IRMS. A correction method for the effects of the added derivative carbon atoms on measurements of the derivatized compounds is also described. This method appears reproducible and allows the detection of 13C enrichment as low as 0.01 mol.%. A practical application for the measurement of 13C plasma lactate enrichment after ingestion of an oral glucose load (0.5 g kg-1) enriched with 0.1% D-(U-13C)glucose is shown.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200221207
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