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  • 1
    Electronic Resource
    Electronic Resource
    Continuous regeneration of NAD(P)+ by flavins covalently bound to sepharose (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1145-1159 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Various flavins, FMN, FAD, and acriflavin, were immobilized to Sepharose using several different coupling methods. The only product stable enough to permit extended studies was acriflavin coupled to epoxy-substituted Sepharose. The nonenzymic oxidizing capacity towards NAD(P)H was investigated and a 25% specific activity, compared to that of free acriflavin, was observed. The reduced acriflavin was immediately auto-reoxidized in air and could thus be reused. It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). The amount of expensive coenzyme necessary for high product formation of such systems was thereby markedly reduced.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260180810
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  • 2
    Electronic Resource
    Electronic Resource
    Steroid transformation by activated living immobilized Arthrobacter simplex cells (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 1267-1284 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A preparation of living Arthrobacter simplex cells immobilized in polyacrylamide gel, which showed steroid-Δ1-dehydrogenase activity, was studied. The entrapped microorganisms catalyzed the transformation of cortisol to prednisolone and this reaction was followed spectrophotometrically or with the aid of thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). About 40% of the original activity found with free bacteria was retained after immobilization. The steroid dehydrogenase activity of polyacrylamide-entrapped A. simplex could be raised to a minor extent in alcoholic solvents or by addition of a cofactor such as menadione. On incubation in various nutrient media, on the other hand, the activity could be increased considerablyl, usually 7-10 times. Possible causes for the observed increase in activity have been investigated, and microbial growth of the original entrapped microorganisms appears to be the major reason. Frozen activated preparations of immobilized A. simplex showed only a small loss of activity on storage for at least four months. A semicontinuous batch wise operation with immobilized A. simplex in different nutrient media was carried out. At the end of the experiment the steroid transformation capacity was 0.5 g steroid per day per g gel (wet weight).
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260200811
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  • 3
    Electronic Resource
    Electronic Resource
    Zur Biosynthese von Flechtenstoffen, Produkten einer symbiotischen Lebensgemeinschaft (1969)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 81 (1969), S. 233-244 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Flechten setzen sich aus Algen und Pilzen zusammen, die miteinander in Symbiose leben. Sie bilden charakteristische, oft polycyclische Flechtenstoffe, welche nur in wenigen Fällen auch in anderen Organismen nachgewiesen werden konnten. Die biosynthetischen Fähigkeiten der Flechten setzen sich nicht additiv aus denen ihrer Bestandteile zusammen. Die in diesem Aufsatz besprochenen Erkenntnisse über die Biosynthese der Flechtenstoffe wurden meistens an 14C-markierten Verbindungen gewonnen.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19690810702
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  • 4
    Electronic Resource
    Electronic Resource
    Biosynthesis of Lichen Substances, Products of a Symbiotic Association (1969)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 8 (1969), S. 240-250 
    Details
    ISSN: 0570-0833
    Keywords: Biosynthesis ; Lichen substances ; Natural products ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Lichens are composed of algae and fungi living together in symbiotic association and forming characteristic, often polycyclic lichen substances that are rarely encountered in other organisms. While the cultures of the unassociated individual constituents of the lichens can live for an unlimited time some doubt surrounds their viability in nature; their biosynthetic powers are not additive in the lichens. The results concerning the biosynthesis of the lichens that are discussed in the present article were obtained mainly with 14C-labeled compounds.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.196902401
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