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  • 1
    Electronic Resource
    Electronic Resource
    Bioluminescent click beetles revisited (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 31-39 
    Details
    ISSN: 0884-3996
    Keywords: Firefly luciferase ; click beetle luciferases ; bioluminescence spectra ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: In studying beetle bioluminescence in the early 1960s, Dr McElroy and his colleagues found that the Jamaican click beetle, Pyrophorus plagiophthalamus, was capable of emitting different colours of light. They further found that the luciferin substrate used by this beetle was the same as that in the firefly, demonstrating that the different colours of bioluminescence were due to differences in the structure of the luciferases. We have recently cloned cDNAs from this beetle species which code for at least four different luciferases. The luciferases are distinguishable by their different colours of bioluminescence when expressed in Escherichia coli. The sequence differences between these different luciferases are few, so the amino acids responsible for the different colours of emission must also be few. Through the construction of hybrid luciferases, by rearranging fragments of the original cDNA clones, we have identified some of these amino acid determinants of colour.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040110
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  • 2
    Electronic Resource
    Electronic Resource
    Introduction to beetle luciferases and their applications (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 289-301 
    Details
    ISSN: 0884-3996
    Keywords: Firefly luciferase ; click beetle luciferases ; reporter genes ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: All beetle luciferases have evolved from a common ancestor: they all use ATP, O2, and a common luciferin as substrates. The most studied of these luciferases is that derived from the firefly Photinus pyralis, a beetle in the superfamily of Cantharoidea. The sensitivity with which the activity of this enzyme can be assayed has made it useful in the measurement of minute concentrations of ATP. With the cloning of the cDNA coding this luciferase, it has also found wide application in molecular biology as a reporter gene. We have recently cloned other cDNAs that code for luciferases from the bioluminescent click beetle, Pyrophorus plagiophthalamus, in the superfamily Elateroidea. These newly acquired luciferases are of at least four different types, distinguishable by their ability to emit different colours of bioluminescence ranging from green to orange. Unique properties of these luciferases, especially their emission of multiple colours, may make them additionally useful in applications.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040141
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  • 3
    Electronic Resource
    Electronic Resource
    Condensation algorithms for the regular mesh substructuring (1995)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 38 (1995), S. 469-488 
    Details
    ISSN: 0029-5981
    Keywords: finite elements ; substructuring ; static condensation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper describes and analyses the implementation of two static condensation algorithms used to exploit the regular patterns created by two schemes of regular mesh substructuring. Computer resources required by the implementation are assessed in terms of the number of multiplications, the total CPU time, the core and the disk storage requirements. It is discovered that, when implemented for the regular mesh substructuring, the newly proposed condensation algorithm is generally more cost effective than the one proposed by Han and Abel, and modified for the regular mesh substructuring by the present authors.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620380308
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  • 4
    Electronic Resource
    Electronic Resource
    Exploiting the physical symmetry of regular mesh in substructuring analysis (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Communications in Applied Numerical Methods 8 (1992), S. 465-478 
    Details
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new technique of multi-level substructuring based on a regular mesh is presented. The technique takes advantage of the physical symmetry and repetitivity generated by the regular mesh. A special node-numbering/addressing system is developed to exploit the symmetry which results in the stiffness matrices not only being symmetrical about the leading diagonal, but a regular pattern emerging with respect to the other diagonal. The same pattern exists for their inverses. A new condensation scheme is used to take full advantage of the regular patterns. As a result, only about a quarter of the stiffness matrices and their inverses need to be processed, as compared to half when using the conventional method.Based on the technique, a two-dimensional finite-element analysis program FEMSUB has been implemented in the University's VAX network. Through a number of numerical tests, it is discovered that the new technique can result in savings of both core storage and computing time. The savings become more significant when the scale of a problem increases. The investigation indicates that the core storage requirement decreases when using substructures of higher levels. However, for computational efficiency, both the level of substructuring and the number of repeated substructures have an effect. Thus, a dimensionless parameter R (number of substructures used/maximum number of substructures) has been introduced to correlate the effect of the technique on computational efficiency.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1630080706
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