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  • 1
    Electronic Resource
    Electronic Resource
    On the use of laser microprobe mass spectrometry for the analysis of organic biomolecules (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 18 (1989), S. 581-591 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Laser microprobe mass spectrometry has been applied to a variety of organic polyfunctional molecules, covering a wide range of polarity and mass spectrometric behaviour. The technique apparently combines desorption under relatively soft conditions with extensive fragmentation and hence allows much structural information from intactly released thermolabiles to be obtained. The mass spectra appear unfamiliar in comparison to conventional techniques. Interpretation is attempted in a purely empirical way by means of the evidence from our database and tentative hypotheses to rationalize the desorption and ionization by laser microbeam irradiation of organic solids. Selected examples are presented to illustrate the potential and limitations of the method in the field of biomolecules, such as pyridoxine and pyridoxal phosphate, nucleosides, nucleotides and related analogues, drugs and the corresponding N-oxides.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200180813
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical ionization desorption mass spectrometry as an additional tool for the structure elucidation of nucleosides (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 377-380 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Promising results were obtained by chemical ionization desorption mass spectrometry for a series of original pyridinium nucleoside salts, together with some purine and pyrimidine nucleo(t)sides. They were recorded using ammonia as reagent gas and tungsten wires. Principal ions correspond to protonated or cationized ([NH4]+) forms of the molecular ion or the free base depending upon the compound under investigation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070904
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  • 3
    Electronic Resource
    Electronic Resource
    Direct liquid introduction LC/MS microbore experiments for the analysis of nucleoside material present in human urine (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 241-245 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to obtain detection limits low enough for the analysis of nucleoside material in biological samples, a direct liquid introduction (liquid chromatographic/mass spectrometric DLI) system was upgraded by inserting a self-built desolvation chamber between the DLI probe and the ion source and by switching to microbore high-performance liquid chromatography (HPLC) on a C-18 column. The system was evaluated for the analysis of pure nucleoside and 2′-deoxynucleoside compounds in CH3OH+H2O (80/20) and for the analysis of nucleoside mixtures which were separated using 0.01 M ammonium formate + methanol (97:3) as eluant. The detection of structurally important fragment ions in the lower mass region which could not be observed before and an enhanced sensitivity are considered to be the main improvements.In combination with an appropriate clean-up procedure the system was evaluated for the DLI liquid chromatographic/mass spectrometric analysis of some nucleosides present in a human urine sample. Pseudouridine and 5,6-dihydrouridine were detected and identified.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200120511
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  • 4
    Electronic Resource
    Electronic Resource
    Mass spectral behaviour of (M - H)- ions of some pyrimidine nucleosides (1993)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1993), S. 419-421 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200220709
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and mass spectrometric characterization of an oxidized form of vasostatin I, an N-terminal chromogranin A-derived protein, from bovine chromaffin cells (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 1599-1604 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: An N-terminal proteolytic processing product of chromogranin A was obtained from bovine chromaffin granules using two steps of C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. Electrospray mass spectrometry revealed a protein with a molecular mass of 8632.0 for the fraction showing immunoreactivity against the N-terminus of chromogranin A, which differed by 48 u from that of the N-terminal processing product, vasostatin I (CGA1-76). Derivatization with mercaptoethanol showed that the peptide had an intact S—S bridge, which is a key structural feature of vasostatin I. Peptide mapping experiments involving reduction/alkylation with vinylpyridine and trypsin digestion were consistent with oxidation of the three methionine residues of vasostatin I to their sulphoxide forms. The oxidation of the methionine residues was found to occur during the C18 solid-phase extraction procedure. The use of freshly prepared Tris-HCl buffer and eluents and flushing the buffer and eluents with nitrogen were shown to result in the isolation of the non-oxidized form of vasostatin I.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190301113
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  • 6
    Electronic Resource
    Electronic Resource
    Thermospray liquid chromatography-mass spectrometry of the DNA adducts formed between 2′-deoxynucleosides and bisphenol A diglycidyl ether (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 1453-1461 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The adducts formed between 2′-deoxyadenosine (dAdo), 2′-deoxycytidine (dCyd), 2′-deoxycytidine (dUrd), 2′-deoxyguanosine (dGuo) and bisphenol A diglycidyl ether (BPADGE) were analysed by high-performance liquid chromatography (HPLC) and thermospray liquid chromatography-mass spectrometry. An HPLC method was developed using a 5RP8 select B column (125 × 4 mm i.d.) and a mobile phase of MeOH/0.1 M NH4OAc at a flow-rate of 0.8 ml min-1. A sample enrichment procedure using solid phase extraction was developed and was found to result in an enrichment factor with a mean value of 9. Mono-adducts were characterized by intense [BH + H]+, [BH + Na]+ and [S]+ ion signals. Imidazole ring opening was observed for the BPADGE/dGuo adduct indicating that the alkylation occurs at N-7. The alkylation site in the BPADGE/dCyd adduct was assigned to N-3 because a hydrolytic deamination reaction was observed leading to the BPADGE/dUrd adduct. Crosslinking was not observed.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190301011
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  • 7
    Electronic Resource
    Electronic Resource
    Mass spectra of 4-substituted-1,2,6-trimethyl-3,5-diethoxycarbonyl-1,4-dihydropyridines (1975)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1975), S. 854-858 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectra of 4-substituted-1,2,6-trimethyl-3,5-diethoxycarbonyl-1,4-dihydropyridines have been studied. The detailed fragmentation mechanisms, supported by high resolution measurements, the study of metastable ions and the use of deuterium labelling, are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210101008
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  • 8
    Electronic Resource
    Electronic Resource
    Liquid chromatography mass spectrometry of nucleosides using a commercially available direct liquid introduction probe (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 347-351 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Mass spectra were obtained for a series of purine and pyrimidine nucleosides in liquid chromatographic mass spectrometric experiments using a direct liquid introduction probe as interface. Separation of a mixture of adenosine, thymidine, uridine and inosine, and a mixture of hypoxanthine, xanthine, adenine, adenosine, uridine, uracil and thymidine was accomplished using gradient elution with methanol and ammonium formate as a direct liquid introduction liquid chromatographic mass spectrometric compatible solvent system.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100602
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