ISSN:
1040-7685
Keywords:
open tubular supercritical fluid chromatography-mass spectrometry
;
ammonia chemical ionization
;
electron ionization
;
direct fluid introduction interface
;
cryopumping
;
Ultramark 3200
;
perfluorinated polyether
;
sucrose polyester
;
SPE
;
olestra
;
noncaloric fat replacement
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Open tubular supercritical fluid chromatography (SFC) with flame ionization detection (FID) has been used for characterizing olestra, a mixture of fatty acid sucrose esters. In this publication, we describe SFC/mass spectrometry (SFC/MS) of olestra using the direct fluid introduction interface. Chemical ionization spectra were used in confirming SFC/FID peak identities. Electron ionization SFC/MS of olestra was also investigated. Both major and minor component peaks in the SFC chromatogram of olestra were characterized. A slight modification of the interface-probe tip was necessary for operation at higher temperature. A procedure was developed for tuning and calibrating the instrument in the 10-4000-u mass range, chemical ionization mode using Ultramark 3200, a perfluorinated polyether. The use of additional pumping in the form of liquid nitrogen cooled cryopumps was key in providing good signal-to-noise ratios for the latereluting (i.e., eluting at higher mobile phase pressures and flow rates) olestra components.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220040402
Permalink
