ISSN:
1040-0397
Keywords:
Aspartate
;
Glutamate
;
Biosensor
;
Flow-Analysis
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Electrochemical enzyme cells for glutamate and aspartate analysis have been assembled and used in continous-flow and in flow-injection analysis. The enzymes, glutamate oxidase (GOD) and aspartate amino-transferase (AST), were co-immobilized on an Immobilon Immuno-affinity membrane, which was placed into a three-electrode, flow-through cell. Additional protective membranes were used to reduce the electrochemical interferences and to protect the immobilized enzymes. Thin-layer and walljet cells were used and compared. Calibration curves for glutamate and aspartate showed a linearity range of 1-500 μmol/L and 10-500 μmol/L respectively. The sensitivity was higher using the flow-through system with a response time of 2 min. Better reproducibility was achieved using the FIA procedure with a response time of less than 1 min. Glutamate in foodstuffs and asparatate in some pharmaceutical products were analyzed. Results were compared and correlated well with an HPLC procedure.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140040905
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