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  • Inorganic Chemistry  (2)
  • Genetics  (1)
  • Wiley-Blackwell  (3)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1265-1274 
    ISSN: 0749-503X
    Keywords: epitope ; tag ; PCR ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Epitope tagging is the insertion of a short stretch of amino acids constituting an epitope into another protein. Tagged proteins can be identified by Western, immunoprecipitation and immunofluorescence assays using pre-existing antibodies. We have designed vectors containing the URA3 gene flanked by direct repeats of epitope tags. We use the polymerase chain reaction (PCR) to amplify the tag-URA3-tag cassette such that the ends of the PCR fragments possess homology to the gene of interest. In vivo recombination is then used to direct integration of the fragment to the location of interest, and transformants are selected by their Ura+ phenotype. Finally, selection for Ura- cells on 5-fluoro-orotic acid plates yields cells where recombination between the repeated epitopes has ‘popped out’ the URA3 gene, leaving a single copy of the epitope at the desired location. PCR epitope tagging (PET) provides a rapid and direct technique for tagging that does not require any cloning steps. We have used PET to tag three Saccharomyces cerevisiae proteins, Cln1, Sic1 and Est1.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 481 (1981), S. 107-116 
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Electrochemical Syntheses. VIII. Preparation of π-Cyclopentadienyl Metal CompoundsThe electrochemical synthesis of π-cyclopentadienyl metal compounds M(π-Cp)2 (M = Fe, Co, Ni) and of cyclopentadienyl carbonyl derivatives (π-Cp)nM(CO)m(M = Fe, Co, Ni, Ti, V) has been investigated. Most of the complexes can be prepared directly from the corresponding metal anodes, monomeric cyclopentadiene (and CO) in moderate or good yields.
    Notes: Die elektrochemische Synthese von π-Cyclopentadienylmetallverbindungen M(π-Cp)2 (M = Fe, Co, Ni) und von Cyclopentadienylcarbonyl-Derivaten des Typs (π-Cp)n-M(CO)m (M = Fe, Co, Ni, Ti, V) wurde näher untersucht. Die Mehrzahl der Komplexe läßt sich direkt aus den entsprechenden Metallanoden, monomerem Cyclopentadien (und CO) in mäßigen bis guten Ausbeuten darstellen.
    Additional Material: 3 Tab.
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 42 (1909), S. 1285-1296 
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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