ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
Deferred standard overcomes problems created by widely differing response factors
;
Deferred standard valuable alternative to internal standard in quantitation
;
Process liquid chromatography facilitated
;
Automatic check on liquid chromatograph
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Among the methods of quantitation commonly used in chromatography, the internal standard may be considered as the most useful and accurate; however, use of internal standards in liquid chromatography with UV and fluorometric detectors runs into considerable difficulties because the response factors may differ from each other by several orders of magnitude. Under these conditions, the search for an IS with a response factor similar to that of the analyte and adequately resolved from other peaks may prove very tedious.The deferred standard concept, already used in process and laboratory gas chromatography, is proposed as a solution; its definition, details of instrumentation, operation, and results are described in this paper. The material presented demonstrates its ability to solve this specific problem. In addition, use of the deferred standard approach is seen to save time and money.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240050304
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