ISSN:
0173-0835
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.
Zusätzliches Material:
1 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/elps.1150030313
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