ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150030313
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