Publication Date:
2012-04-15
Description:
GdX (also named Ubl4A ) is a house-keeping gene located on the X chromosome and encodes a protein harboring an ubiquitin-like domain in human and mouse. Although identified in 1988, the function of GdX remains unknown. To elucidate the role of GdX in vivo, we generated a conditional GdX knockout mouse in which Exon 2 was flanked by two loxP sites. We obtained viable and fertile mice with homozygous GdX flox/flox or GdX flox/Y allele. Germ-line transmission was confirmed by crossing the mouse bearing conditionally targeted allele with an EIIα-Cre transgenic mouse. GdX was successfully depleted in tissues of EII α- Cre - GdX -null mice. GdX −/− and GdX −/ Y mice are viable and exhibit normal development compared with wild-type littermates within 6 months during our observation. We also observed that GdX knockout male mice were functionally normal in the reproductive system where Ubl4B was specifically expressed. GdX flox/flox and GdX flox/Y conditional mice provide a tool for further tissue-specific function analysis of the GdX protein under different conditions. genesis 00:1–9, 2012. © 2012 Wiley Periodicals, Inc.
Topics:
Biology
,
Medicine
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