Publication Date:
2018-01-07
Description:
MicroRNA (miR)-494 has been identified as a predictor and inhibitor in pancreatic cancer. This study aimed to explore the role of miR-494 in pancreatic cancer cells, and the regulation of glioma-associated oncogene 3 (Gli3) by miR-494. The mRNA level of Gli3 in 99 pairs of pancreatic cancer and correspondingly adjacent tissues was monitored by qRT-PCR. Correlation of Gli3 expression with miR-494 level was assessed by Pearson χ2 test. Dual-luciferase reporter assay was used to detect whether Gli3 was a target of miR-494. Following miR-494 mimics and miR-494 inhibitor transfection, the changes in cell viability and migration were detected by using CCK-8 and Transwell chamber. Furthermore, Gli3 siRNA was co-transfected with miR-494 inhibitor, and then cell viability and migration were redetected. Result showed that, the mRNA level of Gli3 in tumor tissues was higher than in the adjacent tissues ( P 〈 0.01). There were 45 in 99 patients with pancreatic cancer expressed Gli3, and significant correlations were observed between the Gli3 level and vascular invasion ( P = 0.04), distant metastasis ( P = 0.001), and histologic grade ( P = 0.03). Gli3 was a direct target of miR-494 ( P 〈 0.01) and it was negatively related by miR-494 ( P 〈 0.01). Overexpression of miR-494 suppressed PANC-1 cells viability ( P 〈 0.05, P 〈 0.01 or P 〈 0.001) and migration ( P 〈 0.01). Additionally, Gli3 silence suppresses miR-494 suppression-induced cell viability and migration ( P 〈 0.01). In conclusion, these data demonstrate miR-494 exhibits tumor-suppressive effects on pancreatic cancer, possibly via targeting Gli3. This article is protected by copyright. All rights reserved
Electronic ISSN:
0091-7419
Topics:
Biology
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Chemistry and Pharmacology
,
Medicine
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