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  • 11
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25502 | 18721 | 2018-10-05 07:27:37 | 25502 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Influx of metallic pollutants such as iron and nickel into marine environment has been increased drastically in current era. Due to its harmful effects on living organisms, the aim of current thesis was isolation and identification of microorganisms which could reduce iron and nickel. In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Imam Khomeini Port cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel and incubated for 24 hours in 30 oC. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Growth curve of selected strains in the presence and absence of iron and nickel were assessed by turbidity method. Along with above mentioned process, removal amount of metal by living cells and dead biomasses of selected strains were assessed by atomic absorption analysis and then 16S rRNA method was used to identify and approve the selected strains. Results of the current study were demonstrated that among of 16 and 12 resistant strains to nickel and iron, two selected strains (number16 and 7) as the most resistant strains had MIC and MBC (29800 and 29700) and (26300 and 26500), respectively. It should be mentioned that iron’s MIC was not assessable. Results of atomic absorption analysis was demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain number 16 respectively and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis which has been isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
    Keywords: Ecology ; Pollution ; Iran ; Bandar Emam ; Rehabilitation ; Recovery ; Nickel ; Iron ; Environmental ; Microorganisms
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 60
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  • 12
    Publication Date: 2021-05-19
    Description: Risks assessment of laboratories of National Fish Processing Research Center was done in 2014 and 2015 to determine the risk factors, potential risks and provide action plans to prevent the occurrence of any risks and increasing awareness of staff to safety issues.all laboratories :Chemistry,Microbiology,sensetive assesment and physical were visited and all factors were evaluated.The metod for evaluation was with Failure Mode and Effects Analysis (FMEA) methods. The assessment process was designed and implemented in two phases, the first phase was the current situation of safety indexes, risks identification and distance to laboratory safety parameters included: working environment, physical factors, test methods and the human factors were done, then risk assessment and risk management of identified risks were followed by check list forms. the second phase was based on the findings,control measures and safety guidelines were done and provided. the highest risk score related to Microbiology laboratory with RPN 540 and then chemistry 360 , because of the nature of sensory laboratory was not evaluated and There was no experiments in the physics laboratory and was ignored. After control measures, the risk number of laboratories decreased as follows: Microbiology to 18, chemistry to 12.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Assessment ; Risk ; FMEA ; Laboratory ; Laboratories Risks Evaluation in National Fish Processing Research Center Laboratories ; Safety
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 13
    Publication Date: 2021-05-19
    Description: Influx of metallic pollutants such as iron and nickel into marine environment has been increased drastically in current era. Due to its harmful effects on living organisms, the aim of current thesis was isolation and identification of microorganisms which could reduce iron and nickel. In order to isolate bacterial strains resistant to iron and nickel, deposit samples were collected from five stations in Imam Khomeini Port cultured on BHI agar medium containing 1000 ppm of iron and 50 ppm of nickel and incubated for 24 hours in 30 oC. Endurance threshold of strains isolated to different concentrations of iron (1000- 15000 ppm) and nickel (100- 25000 ppm) in BHI broth medium were evaluated, the most resistant strains were selected and MIC and MBC were determined. Growth curve of selected strains in the presence and absence of iron and nickel were assessed by turbidity method. Along with above mentioned process, removal amount of metal by living cells and dead biomasses of selected strains were assessed by atomic absorption analysis and then 16S rRNA method was used to identify and approve the selected strains. Results of the current study were demonstrated that among of 16 and 12 resistant strains to nickel and iron, two selected strains (number16 and 7) as the most resistant strains had MIC and MBC (29800 and 29700) and (26300 and 26500), respectively. It should be mentioned that iron’s MIC was not assessable. Results of atomic absorption analysis was demonstrated that the highest amount of nickel reduction in a medium with concentration of 194 ppm and 71 ppm were 16.7% and 63% after 72 hours by selected strain number 16 respectively and in a medium containing 158 ppm iron, after 50 hours 8.4% by selected strain 7, while dead biomass of strain 16 reduced nickel to the amount of 40.6% and it reduced 21% and 4% of iron in solutions with 46 ppm and 792 ppm of iron. Results of molecular analysis demonstrated that strain 16 was Bacillus subtilis. In addition, Bacillus subtilis which has been isolated in this study with ability of nickel and iron removal is an appropriate candidate to be used in aquatic environments.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rehabilitation ; Recovery ; Nickel ; Iron ; Environmental ; Microorganisms
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
    Location Call Number Expected Availability
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