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  • 1
    ISSN: 1476-5535
    Keywords: Keywords: human epidermal growth factor; Bacillus brevis recombinants; expanded bed adsorption; fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Recombinant Bacillus brevis which carried an expression plasmid encoding the human epidermal growth factor (EGF) gene on a cryptic high-copy number plasmid, pHT926, extracellularly produced EGF in its biologically active form at a concentration of over 1.5 g L−1 in the culture broth in a 30-L jar fermenter. The culture broth also contained some other EGF compounds, which mainly consisted of oligomeric and polymeric forms with disulfide bonds. We developed a simple purification method for EGF, without prior cell removal from the culture broth, comprising cation exchange expanded bed adsorption followed by ultrafiltration with UF 10 000 and 3000 membranes. The EGF compounds were efficiently separated from the EGF in its native form in the expanded bed adsorption step. With this purification method, only EGF in its native form was recovered from the culture broth, with a yield of nearly 80%, and 90% purity. This efficient and economic system has made it possible to use EGF as a pharmaceutical in the livestock industry.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 24 (1994), S. 888-893 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Controlling the amount of chromium to be deposited on tin and iron substrates is important for the production of special steel sheet for welded can material, partially tin precoated and then fully coated with chromium. The deposition behaviour of chromium on tin and iron substrates was studied by means of the polarization behaviour of tin and iron in a chromium coating bath. For stable deposition, a higher current density is required on tin than on iron due to the higher hydrogen over-voltage on tin. Ageing in air after tin coating and preliminary electrolysis under the potential range of reduction of Cr6+ to Cr0 in a chromium coating bath are effective in facilitating the formation of a cathode film and encouraging the deposition. A study on a continuous coating line showed that the amount of chromium to be deposited on tin and iron can be industrially controlled by a process involving a lower current density in the first step and a higher current density in the second.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 20 (1964), S. 575-576 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Dans les neurones des noyaux cérébelleux des enregistrements intracellulaires ont montré que la stimulation du cortex cérébelleux peut évoquer les mêmes monosynaptiques IPSP que dans le noyau de Deiters.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 20 (1964), S. 295-296 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Chez le chat anesthésié au Nembutal, les réponses évoquées dans les neurones de Deiters par les stimulations antidromiques ont été étudiées au moyen de microélectrodes intracellulaires.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 15 (1959), S. 11-12 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé La ponte deH. echinata est influencée par la lumière, qui est nécessaire pendant deux périodes: d'abord pendant la gamétogenèse et plus tard (après une période d'obscurité) pour déclencher la maturation finale et la ponte. L'effet de la lumière pendant la deuxième période ne suit pas la règle de Bunsen-Roscoe.H. echinata est comparée, en ce qui concerne l'action des divers facteurs, avecSpirocodon etH. epiconcha. La signification des ions de Ca et Mg est examinée.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 20 (1964), S. 515-516 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Des enregistrements obtenus à partir de neurones de Deiters au moyen de micro-électrodes intracellulaires ont montré que la stimulation du vermis du lobe antérieur cérébelleux provoque le potentiel post-synaptique inhibiteur monosynaptiquement.
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  • 7
    ISSN: 1420-9071
    Keywords: Dopamine ; cerebral neocortex ; immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By using an antiserum raised against dopamine bound to bovine serum albumin, thinner dopamine-labeled nerve terminals were visualized immunohistochemically within neocortical areas, in addition to well-documented dopaminergic innervation into the prefrontal and limbic cortices.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 15 (1959), S. 13-14 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Grâce à une méthode perfectionnée de stimulation, nous avons pu démontrer l'existence, prévue par des indications antérieures, du nerf photosensible chez l'oursinDiadema. Nous avons enregistré des mouvements piquants d'après des observations au cours desquelles le nerf radial, partiellement isolé, était stimulé par des diminutions de l'intensité lumineuse, qui affectaient des parties de 10–100 µ de diamètre.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Mineralogy and petrology 45 (1991), S. 69-83 
    ISSN: 1438-1168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Zusammenfassung Gneise der oberen Amphibolit-Fazies im Südteil des Indischen Schildes zeigen lokal Umwandlungen in Adern, and unregelmäßigen Bereichen von orthopyroxen-führenden trockenen Granuliten (“Incipient Charnockites”). In Abhängigkeit von der Zusammensetzung des Ausgangsgesteins werden diese Zonen in Ortho- und Paracharnockite eingeteilt. Sie entwickelten sich in Gesteinen von charakteristischer mineralogischer und chemischer Zusammensetzung zu verschiedenen Zeit-Intervallen durch die tektonisch kontrollierte Zufuhr von kohlendioxid-reichen Fluiden. Unsere geochemischen Untersuchungen an fünf strategisch ausgewählten Steinbruchen zeigen, daß die “Incipient” Charnockite eine nicht verarmte chemische Zusammensetzung und sehr niedrige K/Rb Werte haben. In den Paracharnockit-Lokalitäten, wo Granulitbildung charakterisiert wird durch das Verschwinden von Granat, Biotit und Quartz, aus denen Orthopyroxene gebildet werden, ist Verlust von Rb und Ba und Anreicherung an Ti zu beobachten. Im Gegensatz dazu zeigen die Orthocharnockite eindeutige LILE Anreicherung mit Zunahme von K, Rb, und Ba und Verlust von Ca0. Dies weist auf extensiven Ersatz von Plagioklas in den Gneisen durch K-Feldspat in den Charnockiten durch K-Na-Ca Austausch-Reaktionen mit zugeführten C02-Fluiden hin. Die deutliche Verarmung an Fe, Mg, Ti und P in diesen Gesteinen wird mit zunehmender Auflösung von Hornblende, Biotit, Magnetit und akzessorischem Apatit erKlärt. Unsere Untersuchungen zeigen, daß die Gneis-Granulit Transformation auch im lokalen Maßstab nicht ein isochemisches Phänomen ist, sondern durch charakteristische Elementtransporte charakterisiert wird. Diese unterscheiden sich jedoch deutlich von den geochemischen Trends, die in einigen regional-metamorphen high grade terrains zu beobachten sind.[/p]
    Notes: Summary Upper amphibolite facies gneisses in the southern Indian Shield show local transformation into veins, clots and patches of orthopyroxene-bearing dry granulites (“incipient charnockites”). Depending upon the protolith composition, these desiccated zones are classified into ortho- and para-charnockites and have developed within rocks of distinct mineralogy and chemistry at different time intervals through the structurally-controlled influx of carbon dioxide-rich fluids. Our geochemical investigations at five critical quarry sections indicate that the incipient charnockites have undepleted chemistry and very low K/Rb values. In the paracharnockite localities, where granulite formation is characterized by consumption of garnet, biotite and quartz to produce orthopyroxene, loss of Rb and Ba and enrichment of Ti are observed. In contrast, the orthocharnockite localities show marked LILE enrichment with gain of K, Rb and Ba and loss of CaO, suggesting extensive replacement of plagioclase in the gneisses by K-feldspar in the charnockite through K-Na-Ca exchange reactions with influxing carbonic fluids. The marked depletion in Fe, Mg, Ti and P in these rocks correlates with progressive dissolution of hornblende, biotite, magnetite and accessory apatite. Our study indicates that gneiss to granulite transformation, even if on a local scale, is not an isochemical phenomenon, but attended by distinct element mobilities, although they are contrastingly different from the geochemical trends in some regional high grade terrains.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 84 (1997), S. 32-34 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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