ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Springer  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis (1979)
    Springer
    Molecular genetics and genomics 168 (1979), S. 293-298 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybrid ColE1 plasmids called ColE1-cosλ-guaA or ColE1-cosλ-gal can be efficiently transduced into various E. coli K-12 cells through packaging into λ phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E. coli K-12 mutants. (1) The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cosλ-guaA. (2) Pre-existing hybrid ColE1 plasmids had no effect on the frequency of λ phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. (3) ColE1-cosλ-guaA and ColE1-cosλ-gal DNAs could temporarily but not stably co-exist in E. coli K-12 recA cells. (4) The presence of ColE1-cosλ-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cosλ-guaA about 7-fold. (5) The same ColE1-cosλ-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA + gen function(s) and suggest that ColE1 plasmid itself provides no recA +-like functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271499
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression of the guanine operon of Escherichia coli as analyzed by bacteriophage lambda-induced mutations (1976)
    Springer
    Molecular genetics and genomics 147 (1976), S. 203-208 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were made on two guaninerequiring strains of Escherichia coli isolated independently as a result of insertion of prophage λ into one of the structural genes of the guanine operon. These mutants do not exhibit any detectable guaB function but express the guaA function constitutively at a low level, presumably due to transcription from the pI promoter on the prophage. Various types of plaque-forming gua-transducing phages were generated from these lysogens. The approximate location and the mode of substitution of the gua genes in the phage genome were determined. These results clearly indicate that the gene guaB is located closer to the operatorpromoter region of the gua operon than is guaA, and the gene order is “operator”-guaB-guaA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267572
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cloning bacterial genes with plasmid λdv (1976)
    Springer
    Molecular genetics and genomics 146 (1976), S. 269-274 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fragments ofEscherichia coli DNA carrying genes for β-galactosidase, or for biosynthesis of guanine or biotin were recombined in vitro with λdv DNA. The cloned recombinant molecules recovered from transformedE. coli cells consisted of a biologically functional bacterial DNA fragment and, except for λdv-bio30-7, two λdv monomer units: one of the λdv units was used as the insertion site for the bacterial DNA, whereas the other was intact, and seemed to be responsible for the replication of the recombinant plasmid. The process which gives rise to these recombinant molecules at high frequency from mixtures of monomeric λdv DNA's and bacterial DNA fragments is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00701250
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Packaging of ColE1 DNA having a lambda phage cohesive end site (1978)
    Springer
    Molecular genetics and genomics 159 (1978), S. 39-45 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mechanism of λ phage-mediated transduction of hybrid colicin E1 DNAs of various lengths was studied, and factors influencing the formation of these transducing particles were investigated. The results were as follows: 1) The presence of a cohesive end site of \gl phage (cos\gl) on colicin E1 DNA was essential for packaging of the DNA 2) Packaging of colicin E1 DNAs, which carry cos\gl with molecular sizes corresponding to 68% of that of \gl phage DNA, was observed in the absence of all known recombination functions of E. coli K-12 and of \gl phage. 3) Hybrid colicin E1 DNAs having cos\gl with molecular sizes corresponding to 28% of that of \gl phage DNA were packaged within \gl phage particles as trimers; hybrid DNAs with cos\gl of 40 and 47% of the length of \gl phage DNA were packaged as dimers; and those with molecular sizes of 68% of that of \gl phage DNA were packaged mostly as monomers. These results demonstrated that two factors are essential for the packaging of DNAs within λ phage particles; the presence of cosλ on the DNA molecule and an appropriate size of DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401746
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...