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  • Springer  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    A new class of clear mutants from coliphage 434 hy not complementing C II and C III mutants for lysogenization (1969)
    Springer
    Molecular genetics and genomics 106 (1969), S. 80-88 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Clear mutants which differ from regular C I , C II , CIIIand y mutants have been isolated from phage 434 hy. These mutants resemble C I mutants in plaque and spot phenotype but efficiently complement C I mutants for lysogenization. Like C II mutants, they do not complement authentic C II mutants for lysogenization but in contrast to C II mutants they also fail to complement C III mutants. They map between the lambda-434 non-homology region and Co 1 (aC II mutant). On account of this map position adjacent to C II the mutants of the new type are called C IIa . They arise from phage 434 hy with a frequency comparable to that of C I and C II mutants. Such mutants are also obtained from phage lambda but apparently not from phage b5. C IIa mutants would not fit into a picture of three independently acting cistrons C I , CII, and C III . The hypothesis is presented that C IIa and C II mutants are in the same structural gene. Two possibilities are discussed that would account for the complementation patterns: 1. C IIa mutants may block the expression of gene C III in cis position; or 2. the products of genes C II and C III function through an oligomeric complex they form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332823
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the phase variation in lambda reduced immunity lysogens (1977)
    Springer
    Molecular genetics and genomics 151 (1977), S. 181-187 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two distinct phases characterized by different levels of immunity that appear in some E. coli strains lysogenic for reduced immunity mutants of bacteriophage lambda are identified as single and tandem double lysogens respectively on the basis of DNA-DNA hybridization experiments and the requirement of the phage xis function for the transition from a single to a double, and of the host recA function for the transition from a double to a single lysogen (in a xis - condition). Rim lysogens with a further increase in immunity, containing some 5 copies of the lambda genome per host genome, have also been observed. It is argued that the different levels of immunity are a direct reflection of the CI gene dosage effect. An unexplained finding is that rim single lysogens yield double lysogens with a frequency of near 1% per generation, whereas cured cells fail to appear even at a frequency 100 times lower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338693
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    Paper (German National Licenses)
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