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  • 1
    Journal cover
    Unknown
    Springer | The Royal Swedish Academy of Sciences
    Online: 39.2010 –
    Print: 22.1993 – 38.2009 (Location: A17, Kompaktmagazin, 3/4)
    Publisher: Springer , The Royal Swedish Academy of Sciences
    Corporation: Kungliga Svenska Vetenskapsakademien 〈Stockholm〉 = Royal Swedish Academy of Sciences 〈Stockholm〉
    Print ISSN: 0044-7447
    Electronic ISSN: 1654-7209
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Keywords: Mensch-Umwelt-Beziehungen, Schweden, Umweltforschung, Umweltschutz
    Abbreviation: Ambio
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  • 2
    ISSN: 1572-8854
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The title complex was prepared by the addition of 15-crown-5, dissolved in acetone, to a solution of Y(NO3)3·nH2O in the same solvent. The crystal structure of this complex, determined by single-crystal X-ray diffraction using counter methods, was carried out on a crystal grown by slow evaporation of the reaction mixture at room temperature. [Y(NO3)3(OH2)3]·1.5(15-crown-5) Me2CO crystallizes in the acentric space group, P21, with unit cell dataa=15.900(5),b=16.530(6),c=11.821(5) Å,β=92.12(3)°,D calc=1.53 g cm−3 forZ=4. Each unit cell consists of two dimers of the formula unit. One crown molecule bridges two [Y(NO3)3(OH2)3] units via hydrogen bonding with one water molecule of each. The two remaining water molecules per metal moiety form three hydrogen bonds to another 15-crown-5 molecule and one to an acetone molecule. The average bonding parameters in the nine-coordinate metal complexes are Y-O(NO3)=2.43(4) Å and Y-O(OH2)=2.34(5) Å.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 16 (1976), S. 436-444 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A search was made for lymphocyte antigens associated with resistance or susceptibility to the T-cell lymphoma induced by the herpes virus of Marek's disease (MD), the experimental model for Burkitt's lymphoma of humans. Antisera were produced by reciprocal immunization with whole blood between an MD-resistant and susceptible line of chickens compatible at the major histocompatibility complex (MHC), and were tested against lymphocytes of both lines. The lymphocytes were not agglutinated, immobilized, or lysed, but their ability to evoke graft-versus-host (GVH) splenomegaly was reduced. This inhibitory activity was line-specific, and these sera had a maximum limiting effect on GVH splenomegaly at a dilution of 1/50 and a minimum at 1/800 dilution. A test based on the differential limitation of GVH splenomegaly by a pair of alloantisera was used to identify the antigens in F1 and F2 generations. The segregation results established a locus,Ly-4, with two codominant alleles,Ly- 4a andLy-4 b .Ly-4 is distinct from theA, B, orC blood group loci and from theBu-1 locus determining B-cell antigens, but may be linked to theTh-1 locus determining T-cell antigens (recombination frequency of 32 percent). Tentative evidence was obtained from comparisons of homozygous F2 and F3 progeny for association of theLy-4 allele characteristic of the susceptible line with increased incidence of MD.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Immunoreactivity for NK1 receptors is confined to specific nerve cell bodies in the guinea-pig ileum, including inhibitory motor neurons and secretomotor neurons. In the present work, endocytosis of NK1 receptors in these enteric neurons was studied following addition of substance P (SP) to isolated ileum. NK1 receptors were localised with antibodies against the C-terminus of this receptor. Some preparations were incubated with SP tagged with the fluorescent label, Cy3.18, so that the fate of SP bound to receptors could be followed. Preparations were analysed by confocal microscopy. In tissue that was incubated at 4° C in the absence of SP, most NK1 receptor immunoreactivity (IR) was confined to surface membranes of nerve cells. At 37° C in the presence of 10−7 M SP (plus 3×10−7M tetrodotoxin to prevent indirect activation via other neurons) the neuronal NK1 receptor was rapidly internalised. After 5 min, NK1 receptor IR was partially internalised, at 20 min NK1 receptor IR was throughout the cytoplasm and in perinuclear aggregates and at 30 min it was again at the cell surface. SP-induced NK1 receptor endocytosis was inhibited by the specific NK1 receptor antagonist, SR140333. Cy3-SP was colocalised with NK1 receptor IR and was internalised with the NK1 receptor. These results show that enteric neurons exhibit authentic NK1 receptors that are rapidly internalised when exposed to their preferred ligand.
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  • 6
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Immunoreactivity for NK1 receptors is confined to specific nerve cell bodies in the guinea-pig ileum, including inhibitory motor neurons and secretomotor neurons. In the present work, endocytosis of NK1 receptors in these enteric neurons was studied following addition of substance P (SP) to isolated ileum. NK1 receptors were localised with antibodies against the C-terminus of this receptor. Some preparations were incubated with SP tagged with the fluorescent label, Cy3.18, so that the fate of SP bound to receptors could be followed. Preparations were analysed by confocal microcopy. In tissue that was incubated at 4° C in the absence of SP, most NK1 receptor immunoreactivity (IR) was confined to surface membranes of nerve cells. At 37° C in the presence of 10−7 M SP (plus 3×10−7M tetrodotoxin to prevent indirect activation via other neurons) the neuronal NK1 receptor was rapidly internalised. After 5 min, NK1 receptor IR was partially internalised, at 20 min NK1 receptor IR was throughout the cytoplasm and in perinuclear aggregates and at 30 min it was again at the cell surface. SP-induced NK1 receptor endocytosis was inhibited by the specific NK1 receptor antagonist, SR140333. Cy3-SP was colocalised with NK1 receptor IR and was internalised with the NK1 receptor. These results show that enteric neurons exhibit authentic NK1 receptors that are rapidly internalised when exposed to their preferred ligand.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 2 (1975), S. 101-130 
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary B identifies a system of two or more loci which have major effects on hostversus-graft and graft-versus-host reactions. LikeH-2 andHL-A, B encodes lymphocyte antigens which react with alloantibodies and allogenic lymphocytes. Like the mammalian MHCs,B has many alleleic variants. LikeH-2 alleles, some of these are associated with specific differences in immune competence and susceptibility to viral oncogenesis. UnlikeH-2, there is no evidence thatB histocompatibility andB serology represent separable genetic systems. In addition,B is associated with functional differences unknown in mammals. These include a general difference in immune competence and differences in the frequency of herpesvirus lymphoma and in reproductive and general fitness. We conclude that the different kinds of information available forB and the mammalian MHCs reflect differences in circumstance, sampling, and technique, and should be regarded as less fundamental than the immunogenetic similarities. We suggest thatB is the phylogenetic homologue ofH-2 andHL-A.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-2932
    Keywords: suspended sediments ; deposition ; granular substrate ; gravel bed ; filtration ; rivers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We present results Irom an experimental study of suspended particle (4.5–36.5 μm silicon carbide powder deposition from surface water to ‘clean’ equi-granular permeable beds in a small 12.5×12.5×15cm box and a re-circulating flume. Enhanced deposition rates of up to 5 times the accepted sediment deposition model (e.g., Einstein,1968 are explained by filtration of particles in the bed. Compared to this model depotiion increases with increasing surface fluid speed, decreasing suspended particle size and increasing bed particle size. These results can be explained by an increased ability of particles to penetrate into the bed with the pore water which increases the effective filter thickness. The predominant deposition mechanism within the bed pores appears to be settling. Enhanced deposition, evident in Einstein's (1968) experimental data, was previously attributed to flocculation but may be explained better by filtration. These enhanced deposition rates drop off to close to the accepted model predicted rate after a certain volume of sediment has entered the bed, which may be due to the stumping of deposits from the top of bed elements. This reduction in deposition rate occurs long before the bed is filled with fine sediment.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0878
    Keywords: Key words Enteric nervous system ; Enteric reflexes ; Intestine ; Tachykinins ; Substance P ; Neurotransmitters ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37°C in Krebs physiological saline containing 3×10–6 M nicardipine, with or without agitation of the villi by gas bubbles. Gut segments were fixed after 0–75 min and processed for immunohistochemistry to reveal the NK1 receptors, following which cells were imaged by confocal microscopy. Initially, receptors were located on the surface and in the cytoplasm of myenteric neurons. In gut incubated without movement of the villi, NK1 receptors returned to the cell surface. After 45 and 60 min, NK1 receptors were detected almost exclusively at the cell surface of 83% and 97% (respectively) of nerve cells that were immunoreactive for NK1 receptors and only 12%–13% of the NK1 receptor fluorescence was located in the cytoplasm. Following the return of receptor to the cell surface, agitation of the villi caused a new wave of endocytosis of the NK1 receptors in 70%–80% of the NK1 receptor-immunoreactive neurons. The percentage of the NK1 receptor fluorescence that was in the cytoplasm increased more than 2-fold to 27±2% after 15 min villous agitation. Action potential blockade by tetrodotoxin (3×10–7 M) prevented the internalisation of the NK1 receptor in response to villous agitation. The degree of internalisation caused by bubbling was similar to that caused by 2×10–9 M substance P. These results indicate that, when enteric reflex circuits are activated by villous movement, tachykinins are released and cause endocytosis of the NK1 receptor in a subpopulation of myenteric neurons.
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