ISSN:
1573-4919
Keywords:
nuclear deoxyribonucleases
;
ATP-inhibited
;
Drosophila embryos
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract Three Drosophila embryonic deoxyribonucleases, designated den1, den2 and den3, are identified in nuclear extracts separated by glycerol density gradient centrifugation. Den1, removes short products from the 5′-ends of single-stranded DNA or double-stranded DNA with either blunt or 5′-recessed termini. Den2 is inactive with single-stranded DNA and acts as 3′-exonuclease with double-stranded DNA possessing either blunt or 3′-recessed termini. Den3 preferentially uses partial duplex DNA containing single-stranded gap and it catalyzes hydrolysis, in 3′-5′ direction, of one of the shorter strands that flank the gap. Nucleolytic activities of den1, den2 and den3 are inhibited with ATP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006879132158
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