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  • 1
    Digitale Medien
    Digitale Medien
    Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 187-188 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The content of proinsulin mRNA sequences was measured in a cultured cell line established from a transplantable hamster islet cell tumor, by hybridization with proinsulin cDNA. The cells cultured in vitro were found to contain a significant amount of proinsulin mRNA sequences, compared with non-insulin-producing hamster tissues, and seem to be a useful system for the study of insulin gene expression.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01953722
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  • 2
    Digitale Medien
    Digitale Medien
    A new route to l-threo-3-[4-(methylthio)phenylserine], a key intermediate for the synthesis of antibiotics: recombinant low-specificity d-threonine aldolase-catalyzed stereospecific resolution (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 586-591 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A new enzymatic resolution process was established for the production of l-threo-3-[4-(methylthio)phenylserine] (MTPS), an intermediate for synthesis of antibiotics, florfenicol and thiamphenicol, using the recombinant low-specificity d-threonine aldolase from Arthrobacter sp. DK-38. Chemically synthesized dl-threo-MTPS was efficiently resolved with either the purified enzyme or the intact recombinant Escherichiacoli cells overproducing the enzyme. Under the optimized experimental conditions, 100 mM (22.8 g l−1) l-threo-MTPS was obtained from 200 mM (45.5 g l−1) dl-threo-MTPS, with a molar yield of 50% and a 99.6% enantiomeric excess.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530051436
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  • 3
    Digitale Medien
    Digitale Medien
    Microbial production of antibiotic fosfomycin by a stereoselective epoxidation and its formation mechanism (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 394-401 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  Fifteen strains of aerobic bacteria and two actinomycetous strains were isolated from various soil samples, using cis-propenylphosphonic acid as the sole source of carbon. Taxonomic studies showed that these belonged to the Pseudomonas, Alcaligenes, Flavobacterium, Aeromonas, Corynebacterium and Streptomyces genera. Most of these strains produced the antibiotic fosfomycin [(—)-(1R, 2S)-1,2-epoxypropylphosphonic acid] from cis-propenylphosphonic acid by stereoselective epoxidation. Under the culture conditions tested, the production of fosfomycin reached a level of 2.0 mg/ml and 0.75 mg/ml by F. esteroaromaticum IFO 3751 and P. putida IK-8 respectively. Purified fosfomycin was an active stereoisomer and its optical purity was a little higher than that of the synthetic compound. Although the stereoespecificity of each enzyme participating in the epoxidation reaction has not yet been clarified, the epoxide formation proceeded through a bromohydrin intermediate produced by bromoperoxidase, finally yielding fosfomycin by the action of halohydrin epoxidase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530050424
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  • 4
    Digitale Medien
    Digitale Medien
    Gene cloning and overproduction of low-specificity d-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 44-51 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The dtaAX gene encoding a pyridoxal 5′-phosphate (pyridoxal-P)-dependent low-specificity d-threonine aldolase was cloned from the chromosomal DNA of Alcaligenes xylosoxidans IFO 12669. It contains an open reading frame consisting of 1,134 nucleotides corresponding to 377 amino acid residues. The predicted amino acid sequence displayed 54% identity with that of d-threonine aldolase from gram-positive bacteria Arthrobacter sp. DK-38, but showed no significant similarity with those of other known pyridoxal-P enzymes. This gram-negative bacterial enzyme was highly overproduced in recombinant Escherichia coli cells, and the specific activity of the enzyme in the cell extract was as high as 18 U/mg (purified enzyme 38.6 U/mg), which was 6,000 times higher than that from the wild-type Alcaligenes cell extract. The recombinant enzyme was thus feasibly purified to homogeneity by ammonium sulfate fractionation and DEAE-Toyopearl chromatography steps. The recombinant low-specificity d-threonine aldolase was shown to be an efficient biocatalyst for resolution of l-β-3,4-methylenedioxyphenylserine, an intermediate for production of a therapeutic drug for Parkinson's disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002539900301
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  • 5
    Digitale Medien
    Digitale Medien
    Microbial production of antibiotic fosfomycin by a stereoselective epoxidation and its formation mechanism (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 394-401 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Fifteen strains of aerobic bacteria and two actinomycetous strains were isolated from various soil samples, using cis-propenylphosphonic acid as the sole source of carbon. Taxonomic studies showed that these belonged to the Pseudomonas, Alcaligenes, Flavobacterium, Aeromonas, Corynebacterium and Streptomyces genera. Most of these strains produced the antibiotic fosfomycin [(-)-(1R, 2S)-1,2-epoxypropylphosphonic acid] from cis-propenylphosphonic acid by stereoselective epoxidation. Under the culture conditions tested, the production of fosfomycin reached a level of 2.0 mg/ml and 0.75 mg/ml by F. esteroaromaticum IFO 3751 and P. putida IK-8 respectively. Purified fosfomycin was an active stereoisomer and its optical purity was a little higher than that of the synthetic compound. Although the stereoespecificity of each enzyme participating in the epoxidation reaction has not yet been clarified, the epoxide formation proceeded through a bromohydrin intermediate produced by bromoperoxidase, finally yielding fosfomycin by the action of halohydrin epoxidase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218439
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  • 6
    Digitale Medien
    Digitale Medien
    Low-specificity l-threonine aldolase of Pseudomonas sp. NCIMB 10558: purification, characterization and its application to β-hydroxy-α-amino acid synthesis (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 702-708 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Low-specificity l-threonine aldolase, catalyzing the reversible cleavage/condensation reaction between l-threonine/l-allo-threonine and glycine plus acetaldehyde, was purified to homogeneity from Pseudomonas sp. NCIMB 10558. The enzyme has an apparent molecular mass of approximately 145 kDa and consists of four identical subunits with a molecular mass of 38 kDa. The enzyme, requiring pyridoxal- 5′-phosphate as a coenzyme, is strictly l-specific at the α position, whereas it can not distinguish between threo and erythro forms at the β position. Besides the reversible cleavage/condensation of threonine, the enzyme also catalyzes the reversible interconversion between glycine plus various aldehydes and l-β-hydroxy-α-amino acids, including l-β-(3,4-dihydroxyphenyl)serine, l-β-(3,4-met‐hylenedioxyphenyl)serine and l-β-phenylserine, providing a new route for the industrial production of these important amino acids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530051235
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  • 7
    Digitale Medien
    Digitale Medien
    Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 193-200 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The gene dak1 encoding a dihydroxyacetone kinase (DHAK) isoenzyme I, one of two isoenzymes in the Schizosaccharomyces pombe IFO 0354 strain, was cloned and sequenced. The dak1 gene comprises 1743 bp and encodes a protein of 62 245 Da. The deduced amino acid sequence showed a similarity to a putative DHAK of Saccharomyces cerevisiae and DHAK of Citrobacter freundii. The dak1 gene was expressed at a high level in Escherichia coli, and the recombinant enzyme was purified to homogeneity and characterized. The acetone powder of recombinant E. coli cells was used to produce dihydroxyacetone phosphate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530051381
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  • 8
    Digitale Medien
    Digitale Medien
    Influence of exciton-exciton interaction with spin paired charge carriers and exciton-lattice interaction on the surface properties of crystalline solids (1989)
    Springer
    Applied physics 49 (1989), S. 631-639 
    Details
    ISSN: 1432-0630
    Schlagwort(e): 71.35.+z ; 73.90. + f
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract Applying tight-binding approximation and spin pairing of like charge carriers in a pair of excitons created in a lattice, the possibility of forming a bound exciton-exciton state is studied. It is found that, provided there exists strong exciton-lattice interaction, such a bound state may be formed and its energy may lie within the valence band deforming the material into a crystalline solid with no energy gap. Lowering of the energy is calculated in naphthalene and anthracene crystals where some experimental results are known. The excess energy released after the formation of such bound state can be adequate, depending on the material, to desorb neutral atoms or eject of electrons from surfaces.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00616987
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  • 9
    Digitale Medien
    Digitale Medien
    Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding phenylacetaldehyde reductase from styrene-assimilating Corynebacterium sp. strain ST-10 (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 386-392 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The gene encoding phenylacetaldehyde reductase (PAR), a useful biocatalyst for producing chiral alcohols, was cloned from the genomic DNA of the styrene-assimilating Corynebacterium sp. strain ST-10. The gene contained an opening reading frame consisting of 1,158 nucleotides corresponding to 385 amino acid residues. The subunit molecular weight was calculated to be 40,299, which was in agreement with that determined by polyacrylamide gel electrophoresis. The enzyme was sufficiently expressed in recombinant Escherichia coli cells for practical use and purified to homogeneity by three-column chromatography steps. The predicted amino acid sequence displayed only 20–29% identity with zinc-containing, NAD+-dependent, long-chain alcohol dehydrogenases. Nevertheless, the probable NAD+- and zinc-binding sites are conserved although one of the three catalytic zinc-binding residues of the zinc-containing, long-chain alcohol dehydrogenases was substituted by Asp in PAR. The protein contains 7.6 mol zinc/mol tetramer. Therefore, the enzyme was considered as a new member of zinc-containing, long-chain alcohol dehydrogenases with a particular and broad substrate specificity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530051536
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  • 10
    Digitale Medien
    Digitale Medien
    Microscopic theory of desorption of neutral atoms due to high excitonic density at the surfaces of III–V compounds (1990)
    Springer
    Applied physics 51 (1990), S. 427-433 
    Details
    ISSN: 1432-0630
    Schlagwort(e): 72.90 ; 73.20 ; 73.90
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: Abstract A microscopic theory of desorption of neutral atoms from the surface of crystalline GaP is presented. Derived results suggest that bonds are broken at the surface due to high excitonic density, so that a pair of excited holes can get localised on the same bond because such an excited state has much lower energy than that of a free exciton state. Any bond with a pair of holes, instead of covalent electrons, will be broken. Strong exciton-lattice interaction is assumed. It is argued that the mechanism of atomic desorption from surfaces is analogous to that of polymer ablation; and the desorption of neutral atoms increases linearly to super linearly with the increase in laser fluence. This agrees well with experiments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00348385
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