ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Reversed-phase systems
;
Octyl, octadecyl, phenyl and cyano-bonded phases
;
Flavonoids
;
Structure-retention relationships
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Retention of an extended set of flavonoid compounds by octyl, octadecyl, phenyl and cyanopropyl-bonded, reversed-phase columns, with methanol, as the organic mobile phase modifier and acetic acid as the acid modifier is reported. Solvent strengths and useful ranges for both isocratic and gradient elution are determined. Relative retention is found to be independent of methanol volume fraction in the mobile phase for all the columns examined. Correlations between retention on different columns, specific selectivity effects and their dependence on molecular structure are analyzed. Practical applications for separation and identification are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02328515
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