ISSN:
1573-5044
Keywords:
Brassicaceae
;
growth regulator-autonomous callus
;
habituated callus
;
hormone autotrophy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Calli from cotyledon, hypocotyl and radicle explants of Erysimum scoparium (Brassicaceae) were induced and cultured using a MS medium without growth regulators. Calli obtained from cotyledon or hypocotyl explants showed a higher growth rate while radicle-derived calli exhibited poor growth. Cotyledon-derived calli were compact and green-colored while hypocotyl- and radicle-derived calli were friable and with creamy-green pigmentation. Histological analysis was carried out during culture and the development of abundant vascular elements and meristematic nodules were found. Subculture on a growth regulator-free medium during 10 months did not ecrease callus growth.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039948
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