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  • 1
    Electronic Resource
    Electronic Resource
    Phosphate transport, membrane potential, and movements of calcium in rat liver mitochondria (1984)
    Springer
    Journal of bioenergetics and biomembranes 16 (1984), S. 101-113 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondria ; liver ; transport ; calcium ; phosphate ; membrane potential ; uncoupler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The membrane potential and calcium accumulation of mitochondria were followed by ion-specific electrodes in the presence of the proton-donor anions phosphate, acetate, glutamate, and beta-hydroxybutyrate. Phosphate was the only anion which allowed rapid and complete restoration of both the membrane potential and the steady-state extramitochondrial calcium concentration after the uptake of 100–200 nmol calcium per mg protein. If there was no influx of any proton-donor anion, the extent of calcium uptake depended on the intramitochondrial phosphate content. Both the fall of the membrane potential and the increase of the external calcium concentration brought about by a given amount of uncoupler were counteracted by phosphate transported into the mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743043
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  • 2
    Electronic Resource
    Electronic Resource
    The nature of absorbance changes following the addition of ADP to mitochondria: ATP synthesis from intramitochondrial inorganic phosphate (1977)
    Springer
    Journal of bioenergetics and biomembranes 9 (1977), S. 213-221 
    Details
    ISSN: 1573-6881
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The transient absorbance increase induced by ADP in phosphate-loaded respiring mitochondria becomes stable and greatly amplified by inhibitors of phosphate transport. The absorbance changes are sensitive to oligomycin and to aurovertin and their extent is proportional to the amount of ADP added. Simultaneously with the ADP-dependent increase in absorbance the inorganic phosphate and K+-ion content of the matrix decreases. It is concluded that the optical change reflects contraction of the matrix compartment secondary to intramitochondrial solute changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743195
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    Paper (German National Licenses)
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