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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum (1999)
    Springer
    Cellular and molecular life sciences 55 (1999), S. 679-682 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Korean mistletoe; mistletoe lectin II (ML II); concanavalin A chromatography; MALDI-TOF.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050324
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  • 2
    Electronic Resource
    Electronic Resource
    Impact of physical initialization on cloud forecasts (1995)
    Springer
    Meteorology and atmospheric physics 56 (1995), S. 261-273 
    Details
    ISSN: 1436-5065
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Summary The impact of initial data on cloud forecasts by the Florida State University Global Spectral Model (FSUGSM) has been investigated. This work has shown that improving the information content of the initial data by physical initialization has a very strong, positive impact on cloud forecasts. Model spin-up of clouds is considerably reduced. There is an overall better representation of high, middle, low, and total clouds over the tropics and there is a discernible improvement in the prediction of clouds. A strong correlation between cloud shortwave forcing and longwave forcing has been noted in model forecasts with the physically initialized data. This result compares very well with observations from the Earth Radiation Budget Experiment (ERBE).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01030140
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  • 3
    Electronic Resource
    Electronic Resource
    High-level expression of the angiotensin-converting-enzyme-inhibiting peptide, YG-1, as tandem multimers in Escherichia coli (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 71-76 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To produce a large quantity of the angiotensin-converting-enzyme(ACE)-inhibiting peptide YG-1, which consists of ten amino acids derived from yeast glyceraldehyde-3-phosphate dehydrogenase, a high-level expression was explored with tandem multimers of the YG-1 gene in Escherichia coli. The genes encoding YG-1 were tandemly multimerized to 9-mers, 18-mers and 27-mers, in which each of the repeating units in the tandem multimers was connected to the neighboring genes by a DNA linker encoding Pro-Gly-Arg for the cleavage of multimers by clostripain. The multimers were cloned into the expression vector pET-21b, and expressed in E. coli BL21(DE3) with isopropyl β-d-thiogalactopyranoside induction. The expressed multimeric peptides encoded by the 9-mer, 18-mer and 27-mer accumulated intracellularly as inclusion bodies and comprised about 67%, 25% and 15% of the total proteins in E. coli respectively. The multimeric peptides expressed as inclusion bodies were cleaved with clostripain, and active monomers were purified to homogeneity by reversed-phase high-performance liquid chromatography. In total, 105 mg pure recombinant YG-1 was obtained from 1 l E. coli culture harboring pETYG9, which contained the 9-mer of the YG-1 gene. The recombinant YG-1 was identical to the natural YG-1 in molecular mass, amino acid sequence and ACE-inhibiting activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051258
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  • 4
    Electronic Resource
    Electronic Resource
    Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHS-A2 (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 397-405 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chitinase gene (chiA) from Pseudomonas sp. YHS-A2 was cloned into Escherichia coli using pUC19. The nucleotide sequence determination revealed a single open reading frame of chiA comprised of 1902 nucleotide base pairs and 633 deduced amino acids with a molecular weight of 67,452 Da. Amino acid sequence alignment showed that ChiA contains two putative chitin-binding domains and a single catalytic domain. Two proline-threonine repeat regions, which are linkers between catalytic and substrate-binding domains in some cellulases and xylanases, were also found. From E. coli, ChiA was purified 12.8-fold relative to the periplasmic fraction. The Michaelis constant and maximum initial velocity for p-nitrophenyl-N,N′-diacetylchitobiose were 1.06 mM and 44.4 μmol/h per mg protein, respectively. The purified ChiA binds not only to colloidal chitin but also to other substrates (avicel, chitosan, and xylan), but the binding affinity of avicel, chitosan, and xylan is around 10 times lower than that of colloidal chitin. The reaction of ChiA with colloidal chitin and chitooligosaccharides (trimer-hexamer) produced an end product of N,N′-diacetylchitobiose, indicating that ChiA is a chitobiosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000408
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  • 5
    Electronic Resource
    Electronic Resource
    X-ray studies of strontium incorporation into bone mineral in vivo (1968)
    Springer
    Calcified tissue international 2 (1968), S. 6-6 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02065188
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  • 6
    Electronic Resource
    Electronic Resource
    Embryo sac development is affected in Petunia inflata plants transformed with an antisense gene encoding the extracellular domain of receptor kinase PRK1 (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 341-350 
    Details
    ISSN: 1432-2145
    Keywords: Key words Embryo sac development ; Ovule ; Petunia inflata ; Receptor kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In a previous study of the function of a pollen-expressed receptor kinase of Petunia inflata, PRK1, it was found that transgenic plants carrying an antisense-PRK1 gene were unable to transmit the transgene through either the male or, unexpectedly, the female. In this report, the nature of this female phenotype was studied using one of the transgenic plants, ASRK-13. Electron and light microscopic examination of the embryo sac and seed development of ASRK-13 and a wild-type plant revealed that embryo sac development of approximately half of the ovules of ASRK-13 was abnormal. The development of the affected embryo sacs was arrested at the late stages of megagametogenesis. The majority of the affected embryo sacs completed three rounds of mitosis normally, but failed to progress through the maturation stages when cell expansion, nuclear migration, and differentiation take place. The remaining small number of abnormal embryo sacs were arrested at either the four- or eight-nucleate stages. The ovules containing the defective embryo sacs apparently failed to be fertilized, resulting in degeneration of half of the seeds produced by ASRK-13. RNA gel blot analysis suggests that the PRK1 gene is expressed in the ovary, albeit at a much lower level than in the anther. The possibility that the antisense PRK1 gene is responsible for the abnormal embryo sac development is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050108
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  • 7
    Electronic Resource
    Electronic Resource
    Sequence of an S-protein of Lycopersicon peruvianum and comparison with other solanaceous S-proteins (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 256-263 
    Details
    ISSN: 1432-2145
    Keywords: Gametophytic self-incompatibility ; Lycopersicon peruvianum ; Nucleotide and amino acid sequences ; S-alleles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones for an S-allele, designated S5, of the self-incompatibility locus (S-locus) of Lycopersicon peruvianum have been isolated by probing a pistil cDNA library with cDNAs for S-alleles of Petunia inflata and Solanum chacoense. The longest S5-cDNA is 869 bp and contains an open reading frame of 217 amino acids. An alignment of the deduced amino acid sequence of S5-protein with that of the 18 S-proteins from five other solanaceous species is presented. Sequence comparison further refines the primary structural features of the S-proteins previously revealed from comparison of subsets of these sequences. Based on this comparison and evidence presented elsewhere, it is proposed that accumulation of point mutations, and not intragenic recombination, is responsible for the generation of new allelic specificities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197375
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  • 8
    Electronic Resource
    Electronic Resource
    Paracetamol elimination in Chinese and Indians in Singapore (1992)
    Springer
    European journal of clinical pharmacology 43 (1992), S. 81-84 
    Details
    ISSN: 1432-1041
    Keywords: Paracetamol metabolism ; ethnic differences ; glucuronidation sulphate conjugation ; glutathione conjugates ; Chinese ; Indians
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The 24 h urinary excretion of paracetamol and its conjugates after oral administration of about 15 mg/kg was studied in 24 Chinese and 24 Indian healthy young adult male volunteers living in Singapore. The Indians excreted a significantly lower fraction of sulphate conjugate (28.9% compared to 35.9% in the Chinese), and a correspondingly higher fraction as glucuronide conjugate (62.2% compared to 54.5% in the Chinese). There was no difference between the ethnic groups in terms of the urinary recovery of unchanged paracetamol (Indians 3.4%, Chinese 3.6%), glutathione-derived cysteine (Indians 2.3% and Chinese 2.2%) and mercapturic acid (Indians 3.6%, Chinese 3.8%) conjugates. The total fraction of administered paracetamol recovered in the urine was 84.7% and 85.7% in the Indian and the Chinese groups, respectively. Although the total glutathione derived conjugates recovered in the two ethnic groups were similar (Indians 5.9%, Chinese 6%), the values were lower than those reported previously for Caucasians in Scotland (9.3%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02280759
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  • 9
    Electronic Resource
    Electronic Resource
    Dynamic crack propagation analysis using Eulerian-Lagrangian kinematic descriptions (1988)
    Springer
    Computational mechanics 3 (1988), S. 141-155 
    Details
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A dynamic version of the Eulerian-Lagrangian kinematic description (ELD) (Koh and Haber (1986 a) is applied to the analysis of elastodynamic fracture problems. The ELD formulation leads to moving finite element procedures which adjust the mesh to changes in the structural geometry due to crack extension. The use of quarter-point isoparametric finite elements with the dynamic ELD ensures correct modeling of the singular forms in both the strain field and the material velocity field. A two-level mapping is used to describe the Eulerian mesh motion based on the crack-tip motion. This greatly reduces, or eliminates, the need to remesh and interpolate field variables to the new node locations, as is required in transient analyses based on Lagrangian models. Stress intensity factors are computed from numerical evaluations of either the actual dynamic energy release rate for running cracks or the instantaneous virtual energy release rate for stationary cracks. Numerical examples indicate that the ELD accurately models geometric changes due to crack growth and their effect on material motion. Three-dimensional color computer visualization techniques are used to interpret the transient field solutions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297441
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  • 10
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of oral salbutamol controlled-release in Asian patients with asthma (1991)
    Springer
    European journal of clinical pharmacology 41 (1991), S. 495-496 
    Details
    ISSN: 1432-1041
    Keywords: Asthma ; Salbutamol ; Asians ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Using a double blind, double dummy crossover design, single dose and steady state pharmacokinetics of oral controlled release (SCR) salbutamol 4 mg and 8 mg tablets b. d. has been studied in 8 Asian patients. Plasma salbutamol was measured over 12 h. In 8 patients the single dose mean Cmax was 4.2 ng·ml−1 and 7.7 ng·ml−1 after 4 and 8 mg, respectively. In 5 patients the steady state mean Cmax, Cmin and tmax were 8.1 ng·ml−1 and 4.7 ng·ml−1 and 6 h for the 4 mg tablets and 14.1 ng·ml−1 and 7.1 ng·ml−1 and 4 h for the 8 mg tablets. It is concluded that both doses of SCR show features of controlled release and that they produced a relatively constant plasma level of salbutamol in Asian patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00626378
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