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  • 1
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum (1992)
    Springer
    Plant cell reports 11 (1992), S. 257-261 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol has been developed for the in vitro propagation of Mexican Weeping Bamboo through somatic embryogenesis from zygotic embryo explants. Mature seeds and excised embryos were cultured in the light or in the dark on both Murashige and Skoog's and Gamborg's B5 basal media with various supplements. Optimal somatic embryogenesis and plant regeneration were obtained by culture in the dark on Murashige and Skoog's basal medium supplemented with 3 mg/1 2,4-dichlorophenoxyacetic acid, 0.5 mg/1 6-benzylaminopurine and 2.0% sucrose. More than 95% of the germinating somatic embryos developed shoots and roots, and were transferred to soil with 85% success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235077
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  • 2
    Electronic Resource
    Electronic Resource
    Regeneration of shoots from cell suspension-derived protoplasts ofAllium cepa (1995)
    Springer
    Plant cell reports 15 (1995), S. 8-11 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus from pre-selected regenerating lines ofAllium cepa andA. fistulosum were used to initiate cell suspensions. Small clusters of callus selected for greater friability were placed into BDS liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA), and were subcultured biweekly. Rapidly growing, finely dispersed lines were used for protoplast isolation. The highest yields came from 3–4 month old cell suspension lines. Protoplasts were cultured in modified K8P liquid medium. Microcalli recovery depended on the number of weeks the cell suspension had been in culture with highest recovery from 4–5 month old cell suspensions. Microcalli were moved to semisolid media when they were approximately 2 mm in diameter. After 4–6 weeks, embryogenic calli thus recovered were moved to variations of standard onion regeneration media containing picloram and BA. Elongating shoots were obtained from up to 88 % of the microcalli of one line, and 40–50 % of the shoots were further multiplied in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690243
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  • 3
    Electronic Resource
    Electronic Resource
    De novo shoot organogenesis of Pinus eldarica Medw. in vitro (1987)
    Springer
    Plant cell reports 6 (1987), S. 163-166 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedling-derived explants of the Afghan pine, Pinus eldarica, were cultured in a triplicate experiment to produce callus that was serially subcultured for up to three years. Callus was removed at various times and induced to regenerate shoots by de novo organogenesis. The shoot regeneration process involved the identification of four discrete developmental steps, each requiring a separate cultural manipulation. In one case a regenerated shoot was induced to root following an auxin pulse treatment. Induction and limited development of buds in callus derived from mature-tree explants was also achieved. This is the first reproducible system for shoot regeneration from long-term callus cultures of a conifer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268468
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  • 4
    Electronic Resource
    Electronic Resource
    De novo shoot organogenesis of Pinus eldarica Medw. in vitro (1987)
    Springer
    Plant cell reports 6 (1987), S. 167-171 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Semi-thin section microscopy was used to evaluate callus formation and subsequent shoot regeneration in the Afghan pine, Pinus eldarica, as correlated to macro-photography of the same processes. Evidence showed the development of unorganized callus required three to six months. Observations over the following two years of culture revealed that regeneration of buds involved induction of subsurface reorganization in the tissues. Buds emerged through the surface of the callus later during development. Evidence indicated regeneration was de novo in origin and proceeded by the mode of shoot organogenesis. A single shoot was adventitiously rooted with continuous vascular connection between shoot and root.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268469
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  • 5
    Electronic Resource
    Electronic Resource
    Circadian variations in the differential blood counts of mice under constant (LL) and alternating (LD) light periods (1982)
    Springer
    Journal of comparative physiology 149 (1982), S. 307-309 
    Details
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Laboratory mice, offspring of CB6 F1 s, were maintained at a constant temperature of 21.0 ± 1.0° C and in constant light (LL) of 40 lux or under 12 h of light (40 lux) alternating with 12 h of darkness (LD) for 8 consecutive days. During the following 36 h, tail blood was secured from each mouse at 6-h intervals. From those samples, a differential white blood cell count, based on 100 leukocytes, was made for each animal. The relative numbers of eosinophils, basophils, and monocytes did not fluctuate rhythmically under LL or LD. The per cent distributions of lymphocytes and neutrophils did vary with circadian frequency in both LL and LD (Figs. 1, 2) The peak counts of neutrophils were found in blood sampled near midnight; high counts of lymphocytes were made in smears prepared about 6:00 a.m. and 6:00 p.m.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00619145
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of genetic diversity in relation to chromosomal inversions in the malaria mosquito Anopheles gambiae (1995)
    Springer
    Journal of molecular evolution 40 (1995), S. 578-584 
    Details
    ISSN: 1432-1432
    Keywords: Nucleotide diversity ; Inversion evolution ; Linked gene complex ; Hitchhiking ; Selective sweep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The epidemiology of malaria in Africa is complicated by the fact that its principal vector, the mosquito Anopheles gambiae, constitutes a complex of six sibling species. Each species is characterized by a unique array of paracentric inversions, as deduced by karyotypic analysis. In addition, most of the species carry a number of polymorphic inversions. In order to develop an understanding of the evolutionary histories of different parts of the genome, we compared the genetic variation of areas inside and outside inversions in two distinct inversion karyotypes of A. gambiae. Thirty-five cDNA clones were mapped on the five arms of the A. gambiae chromosomes with divisional probes. Sixteen of these clones, localized both inside and outside inversions of chromosome 2, were used as probes in order to determine the nucleotide diversity of different parts of the genome in the two inversion karyotypes. We observed that the sequence diversity inside the inversion is more than threefold lower than in areas outside the inversion and that the degree of divergence increases gradually at loci at increasing distance from the inversion. To interpret the data we present a selectionist and a stochastic model, both of which point to a relatively recent origin of the studied inversion and may suggest differences between the evolutionary history of inversions in Anopheles and Drosophila species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160504
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  • 7
    Electronic Resource
    Electronic Resource
    Evidence for the occurrence of polyamine oxidase in the dicotyledonous plant Medicago sativa L. (alfalfa) (1991)
    Springer
    Plant cell reports 10 (1991), S. 550-554 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyamine oxidase (EC 1.5.3.3) activity has not been detected previously in cells of dicotyledonous plants, although it has been characterized extensively in monocotyledonous plants. Evidence is presented in this report for the occurrence of polyamine oxidase in dialyzed crude extracts of the dicotyledonous plant, Medicago sativa L. (alfalfa). Three enzyme assays were used to quantitate the formation of the three products of the reaction catalyzed by polyamine oxidase. 1-Pyrroline formation was measured colorimetrically as a yellow quinazolinium complex with o-aminobenzaldehyde. Hydrogen peroxide formation was measured spectrophotometrically with a coupled peroxidase assay system by peroxidative oxidation of guaiacol. [3H]1,3-Diaminopropane formation was measured by using [1,8-3H]spermidine as the substrate and separating the radiolabelled reaction product from the substrate by paper electrophoresis. This latter assay provided evidence that a polyamine oxidase of type [EC 1.5.3.3] catalyzed the cleavage reaction between a secondary nitrogen atom and an adjacent carbon of the butyl moiety of spermidine. Significant polyamine oxidase activity was detected in floral tissues, cortex tissues of the root, young leaves, and young germinated seedlings of alfalfa. The occurrence of polyamine oxidase in alfalfa accounts for the formation of the essential substrate, 1,3-diaminopropane, required for the biosynthesis of the uncommon polyamines, norspermidine and norspermine, which we have recently detected in alfalfa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232509
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  • 8
    Electronic Resource
    Electronic Resource
    High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration (1987)
    Springer
    Plant cell reports 6 (1987), S. 105-108 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A successful Agrobacterium-mediated transformation system involving a disarmed Ti plasmid is composed of two stages: transformation of cells and recovery of transformed plants. A tissue transformation system with 34% efficiency was developed using stem segments of the interspecific tomato hybrid Lycopersicon esculentum × L. pennellii. This transformation system emphasizes three factors favoring the recovery of transformed plants: 1) promotion of cell division activity at the inoculation site with kinetin in the incubation medium, 2) promotion of adventitious bud initiation by using organized tissue explants in culture, and 3) application of selection at the shoot development stage of adventitious regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276664
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  • 9
    Electronic Resource
    Electronic Resource
    Berechnung von spezifischen Wärmen zweiatomiger Gase nach der Quantenstatistik (1932)
    Springer
    The European physical journal 78 (1932), S. 791-800 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Zusammenfassung Die spezifischen Wärmen zweiatomiger Gase werden nach der Quantenstatistik berechnet unter Berücksichtigung der verschiedenen Abweichungen der Termstruktur von den einfachen Formeln. Bei der Rotation wird die Multiplettstruktur und die Wechselwirkung von Schwingung und Rotation berücksichtigt, bei der Schwingung die Anharmonizität. Die ausgerechneten Korrektionen sind in den meisten Fällen außerordentlich klein. Zum Schluß wird die Bedeutung der klassischen Freiheitsgrade in der Quantenstatistik kurz erörtert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01342039
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic recombination rather than uniparental disomy suggested as another mechanism by which genetic imprinting may play a role in the etiology of Prader-Willi syndrome (1991)
    Springer
    Human genetics 〈Berlin〉 88 (1991), S. 42-48 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Six Prader-Willi syndrome (PWS) patients with normal karyotypes and their parents were analyzed to determine the nature of the molecular aberrations present in the proximal region of 15q and to determine the parental origin of the aberrant chromosome 15. In addition, the likehood that uniparental disomy plays a significant role in the etiology of PWS patients with normal karyotypes was studied. Restriction fragment length polymorphisms (RFLPs) recognized by seven probes [pML34 (D15S9), pTD3-21, pCGS0.9, pCGS1.1 (D15S10), IR4.3 (D15S11), IR10.1 (DS15S12), p189-1 (D15S13), IR39 (D15S18), and CMW-1 (D15S24)] mapping to the Prader-Willi chromosome region (PWCR) and an additional two probes [pMS1-14 (D15S1); the cDNA of neuromedin B] mapping elsewhere on chromosome 15 were analyzed in the six PWS patients and their parents. Copy number of each locus within the PWCR was determined by densitometry. Molecular rearrangements of the proximal region of 15q were observed in all of the six probands and the origin of the aberrant chromosome 15 when determined was consistently paternal in origin. While data obtained from our six patients does not support the mechanism of disomy, results obtained from three of the six patients show more complex rearrangements hypothesized to have resulted from somatic recombination. These rearrangements have resulted in acquired homozygosity and the lack of a paternal allele at various loci within the PWCR. The presence of only a maternal contribution at certain loci as the result of somatic recombination may be another mechanism by which genetic imprinting plays a role in the presentation of the PWS phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204927
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