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  • 1
    Electronic Resource
    Electronic Resource
    Expression of a chimeric CaMV 35S Bacillus thuringiensis insecticidal protein gene in transgenic tobacco (1992)
    Springer
    Plant molecular biology 20 (1992), S. 539-548 
    Details
    ISSN: 1573-5028
    Keywords: Insect-resistant plants ; Bacillus thuringiensis ; insecticidal crystal protein ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Insecticidal transgenic tobacco plants containing a truncated Bacillus thuringiensis cryIA(b) crystal protein (ICP) gene expressed from the CaMV 35S promoter were analyzed for ICP gene expression under field and greenhouse conditions over the course of a growing season. We present new information on temporal and tissue-specific expression of a CaMV 35S/cryIA(b) gene. Levels of cryIA(b) protein and mRNA were compared in both homozygous and hemizygous lines throughout plant development. Levels of ICP mRNA and protein increased during plant development with a pronounced rise in expression at the time of flowering. Homozygous ICP lines produced higher levels of ICP than the corresponding hemizygous lines. ELISA analysis of different tissues in the tobacco plant showed ICP gene expression in most tissues with a predominance of ICP in older tissue. All transgenic ICP tobacco lines which were studied in the field and greenhouse contained 400 ng to 1 μg ICP per gram fresh weight in leaves from the mid-section of the plant at flowering. The amounts of ICP produced by field lines were directly comparable to levels observed in greenhouse-grown plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040612
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  • 2
    Electronic Resource
    Electronic Resource
    The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L. (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 441-446 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A scheme employing genetic markers obtained by in vitro selection was developed for the stringent isolation of hybrid somatic cells of Nicotiana tabacum. Mesophyll protoplasts that carried two dominant alleles of nuclear genes conferring resistance to the herbicide picloram (pmR1) and the ability to utilize glycerol as the sole source of carbon (Gut) were fused with suspension-culture protoplasts that were marked with the dominant nuclear allele (HuR9) conferring resistance to hydroxyurea. Putative somatic hybrid cell lines were identified by selecting for the Gut and HuR9 markers, followed by an assay for the unselected marker PmR1. Plants regenerated from six of these cell lines were proved to be true somatic hybrids by demonstrating the segregation of each of the three parental markers in the progeny of crosses of those plants with normal seed-derived plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325906
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  • 3
    Electronic Resource
    Electronic Resource
    Chlorate-resistant variants of Nicotiana tabacum L. (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 447-454 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four chlorate-resistant cell lines were selected from cells derived from mesophyll tissue of a dihaploid plant of Nicotiana tabacum L. Two of those cell lines, clr0 and clr19, have a high level of resistance to chlorate, cannot assimilate nitrate and lack NADH-nitrate reductase activity. The other two cell lines, clr15 and clr30, have a lower level of resistance to chlorate, can assimilate nitrate and have an altered level and regulation of NADH-nitrate reductase activity. NADH-nitrate reductase activity was partially restored in mixed extracts of clr0 and clr19 cells. Both clr0 and clr19 cells take up nitrate from the medium. Cells of the clr0 line but not those of the clr19 line lack xanthine oxidase activity. Plants regenerated from each of the cell lines, as well as secondary callus cultures initiated from those plants, exhibit the same phenotype as the primary cell lines with respect to chlorate resistance and nitrate utilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325907
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  • 4
    Electronic Resource
    Electronic Resource
    Chlorate-resistant variants of Nicotiana tabacum L. (1983)
    Springer
    Molecular genetics and genomics 189 (1983), S. 455-457 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In somatic hybrids of each of four chlorate-resistant cell lines of Nicotiana tabacum L., two of which were also nitrate-nonutilizing, and a common chlorate-sensitive parent, nitrate utilization and/or chlorate resistance were found to be recessive traits. Complementation for nitrate utilization was observed in somatic hybrids of the two nitrate-nonutilizing cell lines. No chlorate-resistant or nitrate-nonutilizing seedlings were detected among a large number of progeny resulting from the self-fertilization of a somatic hybrid plant one of whose parents was one of the nitrate-nonutilizing cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325908
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