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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 44 (1957), S. 428-429 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 69 (1966), S. 70-78 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Actinomycin D vermag verschiedene phytochromgesteuerte Reaktionen etiolierter Pflanzen zu hemmen. So wird z. B. die von Rotlicht induzierte Aufrollung des ersten Blattes von im Dunkeln kultivierter Gerste besonders durch Act. D gehemmt, wenn man das Antibioticum ungefähr 80 min nach der Belichtung appliziert; spätere oder frühere Darbietung wirkt weniger oder überhaupt nicht. Die Hemmung wird durch Desoxyguanosin aufgehoben. Unter Berücksichtigung der bekannten Aktionsweise des Actinomycins D bei der DNS-abhängigen RNS-Synthese und der aus der Literatur bisher bekannten Tatsachen über phytochromgesteuerte Reaktionen muß vermutet werden, daß das Phytochrom eng an das DNS gebunden ist (vermutlich auch in Plastiden und Mitochondrien!) und als Repressor-System fungiert. Rotlicht löst diese Bindung und ermöglicht dadurch die Gen-Wirkung.
    Notes: Summary Actinomycin-D inhibits phytochrome-mediated responses of etiolated plants. In the unrolling response of a barley first leaf the inhibition by act. D is greater when the antibiotic is applied 80 minutes after irradiation; earlier or later applications are less inhibitory. Inhibition is relieved by deoxyguanosine applied before or after act. D. Similar effects are found with the plumular hooks of peas and beans. These results suggest that phytochrome-mediated responses involve RNA production on a DNA template. The location of phytochrome in the cell is discussed in relation to its possible association with DNA, especially that of the plastids and mitochondria. Phytochrome may thus act as a repressor of gene sequences involved (for instance) in the removal of etiolation symptoms, red light (660 nm) causing its dissociation from DNA. Far-red radiation may reverse the effects by causing re-association of phytochrome.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 73 (1967), S. 369-375 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The responses of three types of explants of blue lupin leaves are considered: pulvinar explants, consisting of the pulvinar region alone, petiolar explants, consisting of the pulvinar region plus petiole and laminar explants consisting of the pulvinar region plus leaflets. Abscission is accelerated by removal of the leaflets; removal of the petiole has much less effect. Pulvinar explants fail to abscise in darkness but are the first to abscise in the light. This is in accordance with previous evidence of high light sensitivity of the pulvinar region. Kinetin applied directly to the pulvinar region delays abscission, as does kinetin supplied via the transpiration stream. As shown by experiment, this is probably due to transported kinetin reaching the abscission zones of the pulvinar region. The effects of photoperiodic treatments on explants or whole leaves are described. Abscission in the whole leaf is delayed by short daily photoperiods; the delay reaches a maximum with 8 hours light per day. However, abscission is more rapid in continuous light than in darkness. Removal of the leaflets greatly accelerates abscission even in darkness. The pulvinar explant fails to abscise with photoperiods of 4 hours or less; although it appears to have a long day response, preliminary attempts failed to demonstrate that this is a true photoperiodic response (replacement of a long day by a short day together with a light break). The complex responses of leaves and explants to day length lend further support to the hypothesis that light has effects on abscission other than in photosynthesis.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 106 (1972), S. 1-12 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The induction of amylase synthesis in barley aleurone layers by gibberellic acid is most sensitive to Actinomycin D (AM) over a short interval late in the lag phase. The duration of the lag phase may be extended as much as 3 fold by lower temperatures over the range 30° to 15° C. At each temperature the AM sensitive period remains close to the end of the lag phase, the period we have previously determined as the stage less sensitive to temperature. Lack of sensitivity to the inhibitor at other periods is not due to failure to penetrate, or to degradation. AM has no effect on tissue respiration, leucine, uridine or uracil uptake, leucine incorporation, or leucine pool size. At all stages it inhibits uracil and uridine incorporation into RNA. Thus AM probably acts by inhibiting RNA synthesis.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 18 (1999), S. 585-588 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 6
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary (2-Chloroethyl)trimethylammonium chloride (Cycocel or CCC), an inhibitor of gibberellin biosynthesis, when repeatedly supplied to the root systems of balsam (Impatiens glandulifera Royle) plants reduces growth in height and the level of gibberellin-like substances in the bleeding sap that exudes from the stumps of detopped plants. Within twelve hours after a single application of the inhibitor to decapitated field peas (Pisum arvense), there are quantitative and qualitative changes in the gibberellins of the sap compared with those in sap collected over the same period of time from untreated plants. These changes are interpreted in terms of the possible blockage by CCC of normal gibberellin production and diversion of precursors into synthesis of “abnormal” gibberellins.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Planta 73 (1967), S. 357-368 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In blue lupin leaves, each leaflet abscises at an abscission zone situated in the pulvinus at its base. The time to abscission of leaflets of detached leaves is proportional to leaf age. Light accelerates abscission; within certain limits the acceleration is the greater the younger the leaf. At a given concentration, kinetin applied to a single leaflet accelerates leaflet abscission in young leaves kept in darkness, delays it in older ones. There is an interaction between kinetin and light which is dependent also on leaf age and kinetin concentration. The leaf can be considered as consisting of three regions, the petiole, the pulvinar region and the leaflets. The effects of kinetin and of light as well as their interactions depent on the regions of the leaf treated with these agents. Kinetin applied to a leaflet of a young leaf kept in darkness accelerates abscission, but kinetin applied to the pulvinar region of a similar leaf kept in darkness delays abscission. When any part of a leaf is illuminated, abscission is accelerated. The most light-sensitive region of the leaf is the pulvinar region, despite its relatively small area. Acceleration of abscission by light is greatest when illumination of the pulvinar region is combined with illumination of either the leaflets or the petiole. The interaction of light with kinetin is complex. Where the illuminated area includes the pulvinar region, kinetin delays abscission. This effect is most marked in the case where the pulvinar region alone is illuminated and kinetin is applied to a leaflet. Intrafoliar abscission as found in lupin leaves permits study of complex interactions of both distal and proximal stimuli involved in abscission.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Planta 63 (1964), S. 382-392 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Zusammenfassung 1. Die Blutungssäfte vonLupinus albus, Pisum sativum (var. Alderman) undImpatiens glandulifera erwiesen sich als verhältnismäßig reich an Stoffen, die im Zwergerbsen-Mais-I- und Weizenblatt-Standardtest gibberellinartige Wirkungen erkennen lassen. 2. Die Aktivität der unverdünnten und ungereinigten Blutungssäfte wird bei manchen Testen durch die negative Wirkung noch unbekannter Stoffe überdeckt. Es wird vermutet, daß es sich dabei um Inhibitoren handelt, die ihrer Natur nach entweder unspezifisch sein könnten oder aber den Charakter von Antigibberellinen besitzen. 3. Die Blutungssäfte vonLupinus undImpatiens wurden in drei pH-Stufen fraktioniert. Die einzelnen Fraktionen (eine neutrale und zwei saure Stufen) wurden durch absteigende Papierchromatographie aufgetrennt. Als Laufmittel diente 1,5 n NH4OH:n-Butanol (1∶3). Im Zwergerbsentest ließen sämtliche Fraktionen eine Reihe von Aktivitätsgipfeln erkennen. Das Chromatogramm der sauren Fraktionen des Lupinensaftes zeigte auch beim Weizenblatt-Test entsprechende Gipfel. 4. Die tägliche Gibberellin-Belieferung des sprosses durch die Wurzel genügt völlig zur Deckung des Wachstumsbedarfs seiner Internodien. In diesem Zusammenhang wird die morphogenetische Rolle der Wurzel bei der Entwicklung des Sprosses kurz besprochen.
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  • 9
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. A method is described for the extraction and purification of Fraction I protein from Avena sativa L. leaves. 2. The protein possesses ribulose diphosphate carboxylase activity. Chromatography on gels of Sephadex G-200 separates phosphoribulokinase and ribose phosphate isomerase from the carboxylase. 3. The S°20w was calculated to be 18.2, the Stokes radius (determined by gel filtration on a cabibrated column) 74 Å, the molecular weight 5.7×105, and the frictional ratio 1.35. 4. An amino acid analysis is presented. 5. Electron microscope observations of negatively-stained Avena Fraction I protein molecules are compatible with the suggestion that they consist of 24 protomers disposed on the surface of an octahedral shell with 4:3:2 symmetry, and of diameter approximately 105 Å.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 124 (1985), S. 205-212 
    ISSN: 1615-6102
    Keywords: Epicuticular wax ; Patterns ; Prepatterns ; Leaf anatomy ; Stomata ; Eucalyptus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Patterns of arrangement of epicuticular wax platelets are shown in a number of species ofEucalytpus. In some, wax platelets are oriented across the long axes of epidermal cells overlying veins and, in some species, over cells of adjacent stomatal complexes. Among other patterns, radial arrangements around stomata, arrangements in lines at right angles to underlying cell walls and concentric arrays are described. The possibility of the involvement of ectodesmata (ectocythodes) in the location of wax platelets is raised. The pattern of orientation of the platelets is ascribed to a “prepattern”, as yet unobserved, of anisotropic domains in the outer cuticle. It is suggested that this prepattern may consist of regions of molecular anisotropy possibly produced in response to stretching of the cuticle during expansion growth. Wax platelet patterns appear to be restricted to certain taxonomic entities; thus, genetical determinants are also involved in their formation.
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