ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary This paper reports on experiments in which a fluorometer, which permits culture fluorescence to be measured in situ was coupled to a bioreactor. The fluorometer through external light excitement NADH can be made to fluoresce, thus allowing intracellular NADH profiles to be measured in response to extracellular step-changes in substrate concentration. Experiments with baker's yeast and Candida tropicalis as organisms and glucose and hexadecane as substrates are reported. The results suggest different uptake mechanisms depending on cell type and on substrate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00499163
Permalink