ISSN:
1432-0878
Keywords:
Lymphocytes
;
Phytohemagglutinin stimulation
;
Nucleolar organizer region
;
Three-dimensional reconstruction
;
Ultrastructure
;
Guinea pig
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00213724
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