ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (2)
  • Transformation  (1)
  • mitochondrial uncoupling  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don (1998)
    Springer
    Plant cell reports 17 (1998), S. 460-468 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsPinus radiata ; Transformation ; Biolistic ; Embryogenic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average number of stable, geneticin-resistant lines recovered was 0.5 per 200 mg fresh weight bombarded tissue. Expression of the uidA reporter gene was detected histochemically and fluorimetrically in transformed embryogenic tissue and in derived mature somatic embryos and regenerated plants. The integration of uidA and npt II genes into the Pinus radiata genome was demonstrated using PCR amplification of the inserts and Southern hybridisation analysis. The expression of both genes in transformed tissue was confirmed by Northern hybridisation analysis. More than 150 transgenic Pinus radiata plants were produced from 20 independent transformation experiments with four different embryogenic clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050426
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A new selective insecticidal uncoupler of oxidative phosphorylation (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 558-560 
    Details
    ISSN: 1420-9071
    Keywords: Lucilia cuprina ; sheep blowfly ; insecticides ; aryl hydrazone ; oxidative phosphorylation ; mitochondrial uncoupling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new aryl hydrazone structure with high insecticidal activity against the Australian sheep blowfly,Lucilia cuprina, was shown to have a higher activity as an uncoupler of oxidative phosphorylation in insect compared to mammalian mitochondrial preparations. This compound possesses the requirements of other uncouplers in its measured pKa and lipid solubility. However, when compared to a closely related structure with similar physicochemical properties, its insecticidal and insect mitochondrial uncoupling activities are greater and it exhibits decreased mammalian toxicity corresponding to this differential biochemical selectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946702
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...