ISSN:
1432-072X
Schlagwort(e):
Thiobacillus tepidarius
;
Thiobacillus versutus
;
ATP synthesis
;
NAD(P) reduction
;
Thiosulphate oxidation
;
Proton potential (Δp)
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Thiosulphate-dependent reduction of NAD and NADP in intact cells of Thiobacillus tepidarius and T. versutus was severely inhibited or abolished by FCCP at concentrations that did not affect ATP synthesis over the same time scale. Thiosulphate-dependent ATP synthesis in T. tepidarius was abolished by the ATPase inhibitor DCCD, which did not affect NAD or NADP reduction at the concentrations tested. These results indicate that energy-dependent NAD(P) reduction using reversal of electron transfer from cytochrome b or c in thiobacilli is directly driven by the Δp generated by thiosulphate oxidation, and does not require the participation of ATP. While NAD(P) reduction and ATP synthesis are thus both effected by sulphur compound oxidation, there is no obligatory link between them.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00411646
Permalink
