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  • 1
    Electronic Resource
    Electronic Resource
    Tissue- and cell-specific distribution of creatine kinase B: A new and highly specific monoclonal antibody for use in immunohistochemistry (1995)
    Springer
    Cell & tissue research 280 (1995), S. 435-446 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Creatine kinase ; B-subunit ; Monoclonal antibody ; Immunohistochemistry ; Immuno-electron microscopy ; Western blot ; Mouse (C57BL/6) ; Rabbit (New Zealand White)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as well as formaldehyde- or Bouin-fixed, paraffin-embedded sections of human, rabbit, and mouse tissues. Moreover, in the study of cell- and tissue-specific distribution patterns, parallel Western blot analysis and immuno-electron microscopy is possible using this antibody. Our analyses demonstrate that creatine kinase B expression is restricted to a specific subset of cell types in various tissues. In brain, the B-subunit was found only in neurocytes, but not in glia cells. High expression was also observed in inner segments of photoreceptor cells and the outer plexiform layer of the retina, in the parietal cells of the stomach and in gut enterocytes, gallbladder and epithelial cells of the urogenital system. The possible roles of the creatine kinase/phosphocreatine-ATP system in these tissues are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307817
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  • 2
    Electronic Resource
    Electronic Resource
    Treatment of Young Spruce Shoots with SO2 and H2S: Effects on Fine Root Chromosomes in Relation to Changes in the Thiol Content and Redox State (1999)
    Springer
    Water, air & soil pollution 116 (1999), S. 423-428 
    Details
    ISSN: 1573-2932
    Keywords: sulfur ; redox status ; thiol ; chromosome ; glutathione ; Picea omorika
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Three year old spruce trees (Picea omorika) were exposed to 100 and 225 nl l-1 SO2 and H2S for three weeks. The number of chromosomal aberrations and the mitotic index in the root tip meristems, and glutathione and cysteine contents in fine roots were determined twice weekly. An increase in glutathione content in fine roots of H2S exposed plants was only detectable after 13 days of fumigation. The number of chromosomal aberrations increased significantly after 9 days of exposure to 225 nl l-1 H2S and after 13 days of exposure to 225 nl l-1 SO2 or 100 nl l-1 H2S. This increase in chromosomal damage persisted up to the end of the 3 week treatment. Neither SO2 nor H2S exposure affected the cysteine content or the redox state of glutathione in fine roots. These results suggest that the development of chromosomal aberrations during SO2 and H2S exposures does not directly reflect changes in thiol/glutathione content or redox state in the fine roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005275629427
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