ISSN:
1432-2242
Keywords:
Oryza sativa
;
Haploid
;
Nodal segment
;
Colchicine
;
Chromosome doubling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Rice nodal segments from three flowering haploids were excised and treated for different lengths of time with 0.3% or 0.4% colchicine (dissolved in 2% DMSO) in an attempt to induce fertile seeds. A combination of higher colchicine concentration and longer hours of treatment reduced the survival rate of treated segments, but more fertile plants were transformed. Pooled data showed that of the 842 segments used, 42.2% survived the treatment and sprouted, but only 31.9% were successfully established and grown to maturity. Among the 269 mature plants, 29,4% produced fertile seeds (panicles) with an average of 146.2 seeds per diploidized plant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00292330
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