ISSN:
1573-5044
Keywords:
embryoid
;
Helianthus annuus
;
isolation
;
plant donor tissue
;
protoplast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effects of factors influencing sunflower protoplast isolation yield, plating efficiency (PE) and the early differentiation into embryoids (embryogenic capacity, EC) have been studied. Only hypocotyl-derived protoplasts divided. The variations of PE and EC in the various treatments did not seem to be linked to the protoplast yields. From statistical analysis of the data, we concluded that, the sunflower genotype, the age and height of seedlings, the part of hypocotyl used, the incubation time (from 6 to 16 hours) in enzymes of explants or of protoplasts alone, influenced PE but large variations were detected for EC. A comparison of the factors effecting EC suggested an origin, inside the hypocotyl, of cells able to give rise, after induction, to embryogenic protoplasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033634
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