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  • NMR spectroscopy  (1)
  • complementary DNA  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland ofBombyx mori (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1167-1171 
    Details
    ISSN: 1420-9071
    Keywords: Silkworm ; Bombyx mori ; silk gland ; mRNA ; complementary DNA ; fibroin light chain ; molecular cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm,Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951711
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  • 2
    Electronic Resource
    Electronic Resource
    Water soluble cyclodextrin polymers: Their interaction with drugs (1987)
    Springer
    Journal of inclusion phenomena and macrocyclic chemistry 5 (1987), S. 427-431 
    Details
    ISSN: 1573-1111
    Keywords: Cyclodextrin polymer ; solubility method ; complex stability ; NMR spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The complex forming ability of a water-soluble β-cyclodextrin epichlorohydrin, polymer (CDPS) and its different molecular weight fractions was studied and compared with the complexing properties of β-cyclodextrin (βCD) and dimethyl-βCD (DM-βCD). CDPS was separated into two main fractions. CDPS and its fractions formed well soluble inclusion compounds with the studied drugs. The low molecular weight fraction formed rather stable complexes with small guest molecules, the high molecular weight fraction was found to be more efficient in binding larger substrates. Structural studies of furosemide-CD complexes were attempted by NMR spectroscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00664098
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    Paper (German National Licenses)
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