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  • In situ hybridization  (2)
  • residual stress  (2)
  • Springer  (4)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of nondestructive evaluation 8 (1989), S. 235-245 
    ISSN: 1573-4862
    Schlagwort(e): Multi-layer thin film structure ; low-cycle fatigue ; cycle-to-crack ; DC resistance ; dislocation density ; residual stress
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Elektrotechnik, Elektronik, Nachrichtentechnik , Mathematik
    Notizen: Abstract A multi-layered thin film structure (namely, electrodeposited Cu/sputtered Cr/Kapton substrate/sputtered Cr/electrodeposited Cu), utilized as a flexible component for computers, has been exposed to fatigue. Although a standardized testing method for fatigue ductility is available for a solid monolayer of electrodeposited foil, there is no method available for examining such a multi-layered thin film structure. In this study, four different methods were employed to characterize the low-cycle fatigue damage: (1) DC resistance measurement, (2) residual stress development by x-ray diffraction, (3) dislocation density calculation by using obtained x-ray diffraction line profiles, and (4) microscopic observations. Low-cycle fatigue was conducted at eight levels of applied total strain, i.e., δε T =13.95%, 7.69%, 5.83%, 4.69%, 3.37%, 2.37%, 1.59%, and 1.19%. The number of fatigue cycles, when the crack was first observed on the outer Cu layer, was identical to that observed with the onset of increased resistance. This cycle number is thus designated as the number of cycles-to-fatigue crack initiation,N c . AtN c , the residual stresses also show a noticeable relaxation, and the dislocation density shows a remarkable increase. IfN c is plotted against the applied total strain amplitudes, a Manson-Coffin's relationship is obtained with an exponent of 0.39. It is recommended that monitoring the continuous changes in DC resistance could provide a reliable nondestructive evaluation of low-cycle fatigue life of a multi-layered thin film structure.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of nondestructive evaluation 10 (1991), S. 79-87 
    ISSN: 1573-4862
    Schlagwort(e): X-ray diffraction ; tensors ; nondestructive evaluation ; residual stress
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Elektrotechnik, Elektronik, Nachrichtentechnik , Mathematik
    Notizen: Abstract The three-dimensional residual stress condition of copper sputtered on Kapton™ films was studied by two nondestructive x-ray diffraction techniques. Four samples were evaluated—one “control sample,” and three heat-treated samples. Because of concerns about steep stress gradients normal to the specimen surface, an experimental technique described by Dölle(1) was used to obtain the residual strain tensors in the near surface region. The resulting stress tensors were compared to data obtained through a modification of the technique described by Dölle, referred to as the differential method.(2)
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 90 (1995), S. 526-533 
    ISSN: 1432-2242
    Schlagwort(e): Haynaldia villosa ; Repeated sequence ; In situ hybridization ; Gene transfer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A species-specific repeated sequence, pHvNAU62, was cloned from Haynaldia villosa, a wheat relative of great importance. It strongly hybridized to H. villosa, but not to wheat. In situ hybridization localized this sequence to six of seven H. villosa chromosome pairs in telomeric or sub-telomeric regions. Southern hybridization to whea-H. villosa addition lines showed that chromosomes 1V through 6V gave strong signals in ladders while chromosome 7V escaped detection. In addition to H. villosa, several Triticeae species were identified for a high abundance of the pHvNAU62 repeated sequence, among which Thinopyrum bassarabicum and Leymus racemosus produced the strongest signals. Sequence analysis indicated that the cloned fragment was 292 bp long, being AT rich (61%), and showed 67% homology of pSc7235, a rye repeated sequence. Isochizomer analysis suggested that the present repeated sequence was heavily methylated at the cytosine of the CpG dimer in the genome of H. villosa.It was also demonstrated that pHvNAU62 is useful in tagging the introduced 6VS chromosome arm, which confers a resistance gene to wheat powdery mildew, in the segregating generations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Haynaldia villosa ; Alien translocation ; Powdery mildew resistance ; In situ hybridization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Several Triticum aestivum L.-Haynaldia villosa disomic 6VS/6AL translocation lines with powdery mildew resistance were developed from the hybridization between common wheat cultivar Yangmai 5 and alien substitution line 6V(6A). Mitotic and meiotic C-banding analysis, aneuploid analysis with double ditelosomic stocks, in situ hybridization, as well as the phenotypic assessment of powdery mildew resistance, were used to characterize these lines. The same translocated chromosome, with breakpoints near the centromere, appears to be present in all the lines, despite variation among the lines in their morphology and agronomic characteristics. The resistance gene, conferred by H. villosa and designated as Pm21, is a new and promising source of powdery mildew resistance in wheat breeding.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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