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  • 1
    Electronic Resource
    Electronic Resource
    Hypothalamic histamine modulates adaptive behavior of rats at high environmental temperature (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 283-285 
    Details
    ISSN: 1420-9071
    Keywords: Histamine ; hypothalamus ; environmental temperature ; adaptive behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histamine content in the rat hypothalamus was lower at 4°C and higher at 31°C compared to that at 21°C. Pretreatment with α-fluoromethylhistidine, a ‘suicide’ inhibitor of histidine decarboxylase, attenuated both the increased level of hypothalamic histamine and rat adaptive behavior at 31°C. Increase of histamine content in the hypothalamus appears to be an important factor contributing to rat adaptive behavior to high environmental temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951767
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on separation mechanisms of biological molecules on a polyvinyl alcohol column (1984)
    Springer
    Chromatographia 18 (1984), S. 550-555 
    Details
    ISSN: 1612-1112
    Keywords: Liquid column chromatography ; Size-exclusion chromatography ; Reversed-phase chromatography ; Polyvinyl alcohol column
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic separation mechanism on a polyvinyl alcohol (PVA) column in aqueous systems was explored utilizing several different types of compound such as polyethylene glycols, carbohydrates, pyrimidine and purine bases, fatty acids, monophosphate nucleotides and glycyl-peptides. Two types of separation mechanisms were found to occur for these substrates. The polyethylene glycols and the carbohydrates were eluted by size-exclusion chromatography. The retention behavior of the other substrates could be explained by the solvophobic theory, suggesting that the predominant separation mode was reversed-phase chromatography. The occurrence of reversed-phase chromatography was also indicated by the remarkable effect of the addition of ion-association reagents to the chromatographic system on the retention of the monophosphate nucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265693
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of histamine N-methyltransferase-like structures in rat kidney (1986)
    Springer
    Cell & tissue research 243 (1986), S. 681-684 
    Details
    ISSN: 1432-0878
    Keywords: Histamine ; Histamine N-methyltransferase ; Immunohistochemistry ; Kidney ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histamine N-methyltransferase (S-adenosylmethionine: histamine N-methyltransferase, E.C. 2.1.1.8) was purified to homogeneity from rat kidney, and antibody was raised against it in guinea pigs. The antibody immunoprecipitated histamine N-methyltransferase. Immunofluorescent histochemical studies with anti-histamine N-methyltransferase antibody as the first antibody and goat antiguinea pig IgG conjugated with fluorescein isothiocyanate as the second, showed the presence of immunoreactive structures in the proximal tubules of rat kidney. The brain showed no immunoreaction with the antibody.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218079
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  • 4
    Electronic Resource
    Electronic Resource
    Separation of single-stranded oligonucleotides on polyvinyl alcohol gel column (1985)
    Springer
    Chromatographia 20 (1985), S. 713-716 
    Details
    ISSN: 1612-1112
    Keywords: Size-exclusion chromatography ; Polyvinyl alcohol gel column ; Oligonucleotides ; Hydrophobic interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Separation mechanisms for single-stranded oligodeoxyribo-or oligoribonucleic acid fragments were explored on an Asahipak polyvinyl alcohol gel column (GS-320) by use of sequential isomers of such molecules. Substrates having different base numbers were found to be separated by size-exclusion chromatography while those having the same numbers with different base sequences were isolated by use of the reversed-phase mode. By using those dual modes, a limit for the separation of the samples was found to arise because one mode shifted the peaks of the substrates in the sense opposite to the shift resulting from the other mode and it was found that when substrates had less than nine bases, the solutes eluted separately.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02282928
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