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  • Nitrification  (4)
  • H ion efflux  (3)
  • exchangeable Ca  (3)
  • Springer  (10)
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  • Springer  (10)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 24 (1990), S. 173-180 
    ISSN: 1573-0867
    Keywords: Phosphate ; resin beads ; resin membranes ; resin-strips ; anion exchange resin ; cation exchange resin ; P sorption capacity ; exchangeable Ca ; phosphate rocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A simplified procedure for determining the amount of phosphate (P) extracted from soils by ion exchange resin membranes is reported. Strips of anion (HCO 3 - form) and cation (Na+ form) exchange membrane were shaken with suspensions of soil in deionised water for 16–17 hours. After shaking, the strips were thoroughly rinsed in deionised water before the phosphate retained on the anion exchange resin strip was determined by shaking the strip directly with phosphate reagent. Compared to the common use of resin beads in nylon mesh bags, this resin membrane procedure is simpler, more convenient, and because an elution step is omitted, less time consuming. The mixed resin membrane method for soil phosphate extraction was compared to the use of resin bags on four New Zealand soils, contrasting in P sorbing capacity and exchangeable calcium. The soils were preincubated with and without 240 mg P kg−1 soil with three P sources of different solubilities. The resin strips extracted amounts of P which were closely correlated (R2 = 0.972) with that extracted by the resin bags. The amounts of P extracted by the mixed resin procedure were in proportion to the solubility of the P sources in each soil.
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  • 2
    ISSN: 1573-0867
    Keywords: Dry matter yield ; exchangeable Ca ; phosphate rocks ; P sorption capacity ; relative agronomic effectiveness ; substitution ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Six phosphate rocks (PRs) of varying reactivities were compared with monocalcium phosphate (MCP) in a glasshouse experiment growing perennial ryegrass (Lolium perenne cv. Nui) as the test plant on four soils of contrasting P sorption capacity and exchangeable Ca. The cumulative dry matter yield over 10 harvests showed a significant response to P application in all soils. Based on relative yield and P uptake, MCP was the most effective P fertilizer followed by the reactive phosphate rocks, which were superior to the unreactive rocks in all soils. The relative agronomic effectiveness (RAE) and substitution ratio (SR) of individual PR fertilizers, calculated with respect to MCP using the methods of ‘vertical’ and ‘horizontal’ comparison, respectively, were similar over a range of fertilizer rate. There was a decline or slight increase in the performance of PRs with time in the low P sorption soils but a consistent increase in the high P sorption soils. Some initial influence of exchangeable Ca content of the soils on the relative performance of PRs was also observed. Generally the PRs performed better in high P sorption soils than low P sorption soils and in low exchangeable Ca soils than high exchangeable Ca soils.
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  • 3
    ISSN: 1573-5036
    Keywords: Brassica napus ; H ion efflux ; nitrate accumulation ; nitrate reductase ; phosphate nutrition ; xylem exudation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Levels of nitrate reductase activity (N.R.A.) were measured in shoots and roots of P sufficient and P deficient rape plants and changes in N.R.A. examined in relation to the onset of H ion efflux from the roots. Rates of xylem exudation were measured and the sap analysed for nitrate, amino-N and phosphate content. The optimum concentration of phosphate in the leaves for N.R.A. was about 0.7%. Both high and low concentrations of phosphate within the leaves inhibited N.R.A in those leaves. This inhibition of N.R.A led to the accumulation of nitrate in the older parts of the shoots of P sufficient plants. Less accumulation of nitrate occurred in the P deficient plants since nitrate uptake by the plants decreased before any fall in N.R.A. Xylem exudation rates halved within 18 hours of depriving the plants of phosphate, and, since the composition of the sap remained constant, this indicated a reduced flux of nitrate into the xylem. The rate of xylem exudation continued to fall and by the end of the experiment was approximately one tenth of the rate in the P sufficient plants. The onset of H ion efflux from the terminal portions of the root preceded any effect on N.R.A by 2 days.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 105 (1988), S. 247-256 
    ISSN: 1573-5036
    Keywords: Brassica napus ; cation-anion balance ; H ion efflux ; phosphate nutrition ; root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in pH around the roots of young rape plants were studied using a nutrient film technique which allowed either part or all of the root system to be subjected to specific nutrient treatments. The rapidity and direction of change of pH was assessed by embedding absorbing roots in a thin layer of agar containing bromocresol purple. Measurements were also made with a pH microelectrode placed next to the roots. Phosphate-fed plants were deprived of phosphate when 14 days old. Patterns of pH changes round the deprived roots were the same as with phosphate-fed plants until the plants had been deprived of P for three days, when H ion efflux started in the terminal portions of the roots. The lengths of root producing acid and amounts of H ion both increased as the plants became more P deficient. Both P fed and P deprived roots produced HCO3 ions but the net amount of HCO3 ion produced by the P deficient roots fell as did nitrate uptake rates. Cation-anion balances measured at the end of the experiment showed that uptake of all anions and K decreased in the P deprived plants but uptake of Ca and Mg were little altered. This resulted in a smaller ratio of anions to cations absorbed which was reflected in the reduced HCO3 ion efflux.
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  • 5
    ISSN: 1573-5036
    Keywords: Ammonification ; Clay soil ; Exchangeable ammonium ; Grassland ; Incubation ; Kinetics ; Nitrate ; Nitrification ; N cycle ; N mineralization ; Soil Moisture ; Soil temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Net mineralization of N and net nitrification in field-moist clay soils (Evesham-Kingston series) from arable and grassland sites were measured in laboratory incubation experiments at 4, 10 and 20°C. Three depth fractions to 30 cm were used. Nitrate accumulated at all temperatures except when the soil was very dry (θ=0.13 cm3 cm−3). Exchangeable NH4-ions declined during the first 24 h and thereafter remained low. Net mineralization and net nitrification approximated to zero-order reactions after 24 h, with Q10 values generally 〈1.6. The effect of temperature on both processes was linear although some results conformed to an Arrhenius-type relationship. The dependence of net mineralization and net nitrification in the field soil on soil temperature (10 cm depth) and moisture (0–15, 15–25, 25–35 cm depths) was modelled using the laboratory incubation data. An annual net mineralization of 350 kg N ha−1 and net nitrification of 346 kg N ha−1 were predicted between September 1980 and August 1981. The model probably overstressed the effect of soil moisture relative to soil temperature.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 86 (1985), S. 425-439 
    ISSN: 1573-5036
    Keywords: Ammonium ; Chloride ; Growth ; Inhibition ; Monod model ; Nitrification ; Soil ; Sulphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Following the addition of 0–75 μmole N g−1 as ammonium chloride or ammonium sulphate to a sandy loam soil the nitrate formed was measured daily for a period of 15–17 days. The nitrate produced as a function of time was described using the Monod equation for microbial growth. An optimisation technique is described for obtaining, from the nitrification time course data, the maximum specific growth rate, the affinity constantant and an index limited by the concentration of ammonium in soil solution. Additions of more than 7.3 μmoles N g−1 soil as ammonium chloride were found to inhibit nitrification. The inhibition was interpreted as being caused by osmotic pressure or by chloride ion. A similar effect was not found with ammonium sulphate, because the salt concentration in the soil solution was restricted by the precipitation of calcium sulphate. The model developed was capable of accounting for nitrate production in the soil under non-steady state conditions of substrate concentrations and nitrifier biomass.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 84 (1985), S. 403-415 
    ISSN: 1573-5036
    Keywords: Agar-pH indicator technique ; Cation-anion balance ; H ion efflux ; HCO3 ; ion efflux ; Nitrate nutrition ; Nitrate reductase ; Root growth ; Split root system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When nitrate-fed plants were deprived of all sources of nitrogen at 15 or 17 days old, the release of H ions from the roots was immediate and uniformly distributed over the root length. When nitrate was witheld from half of the root system of nitrate-fed plants, the roots deprived of nitrate immediately started to produce H ions even though the nitrate-fed half of the root system continued to supply the whole of the plant with nitrate. However, the rate of H ion production in plants partly supplied with NO3 was less than in plants completely deprived of NO3. It is suggested that malate produced in the shoots, following nitrate reduction, may be redistributed to the roots deprived of nitrate. There, HCO3 produced by the decarboxylation of the malate masks some of the expected H ion efflux.
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  • 8
    ISSN: 1573-0867
    Keywords: Anion exchange resin ; Bray 1 ; cation exchange resin ; Colwell ; exchangeable Ca ; P dissolution ; P fractionation ; P sorption capacity ; P tests ; Phosphate rocks ; Olsen ; Truog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The objective of this work was to develop and evaluate a soil test suitable for estimating the phosphorus status of soils whether they were fertilized with soluble or sparingly soluble P fertilizers or both. Four New Zealand soils of contrasting P sorption capacity and exchangeable Ca content were incubated alone or with monocalcium phosphate (MCP), reactive North Carolina (NC) phosphate rock or unreactive Florida (FRD) rock, at 240 mg P kg−1 soil, to allow the P sources of different solubilities to react with each soil and provide soil samples containing different amounts of extractable P, Ca and residual phosphate rock. The phosphorus in the incubated soils was fractionated into alkali soluble and acid soluble P fractions using a sequential extraction procedure to assess the extent of phosphate rock dissolution. Eight soil P tests [three moderately alkaline — Olsen (0.5M NaHCO3) modified Olsen (pretreatment with 1M NaCl) and Colwell; three acid tests — Bray 1, modified Bray 1 and Truog; and two resin tests — bicarbonate anion exchange resin (AER) and combined AER plus sodium cation exchange resin (CER)] were assessed in their ability to extract P from the incubated soils. The 0.5M NaHCO3 based alkaline tests could not differentiate between the Control and FRD treatments in any soil nor between the Control, NC and FRD treatments in the high P sorption soils. The acid extractants appeared to be affected by the P sorption capacity of the soil probably because of reabsorption of dissolved P in the acid medium. The AER test gave results similar to Olsen. Only the combined AER + CER test extracted P in amounts related to the solubility of the P sources incubated with each soil. Furthermore, when soil samples were “spiked” with FRD and NC and extracted immediately, the P extracted by the AER + CER test, over and above the control soils, increased with the amount and chemical reactivity of the rocks. There was no extraction of rock P by any of the alkaline extractions. Increases in the amounts of P extracted (ΔP) by each soil test from the fertilized soils, over and above the control soils were compared with the amounts ofP dissolved from the fertilizers during incubation (measured by P fractionation). Soil P sorption capacity had least influence on the amounts of P extracted by the AER + CER and Colwell tests. However, the Colwell test was unable to differentiate between all P sources in all four soils and suffered from the disadvantage of producing coloured extracts. The AER + CER test appeared to have the potential to assess the available P status of soils better than the other tests used because of its ability to extract a representative portion of residual PR (in accordance with the amount and reactivity) and dissolved P, and thus to differentiate between fertilizer treatments in all four soils.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 97 (1987), S. 37-45 
    ISSN: 1573-5036
    Keywords: Ammonium ; Chloride ; Inhibition ; Nitrification ; Osmotic pressure ; Sorbitol ; Sulphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A short term nitrification assay (〈18 h) was used to assess the effect of high concentrations of different solutes on the rate of nitrate production. High solute concentrations were found to inhibit nitrification and the degree of inhibition was related both to the osmotic pressure of the soil solution and the osmoticum used. Ammonium chloride, ammonium sulphate and sorbitol were used as sources of osmotic pressure. The results showed that, with ammonium salts, no inhibition was observed with pressures less than 2 atm. Above these values, the severity of the inhibition followed the order ammonium chloride〉ammonium sulphate〉sorbitol up to the maximum osmotic pressure studied (25 atm). With ammonium chloride, a pressure of 3.5 atm. was sufficient to cause a 90% inhibition of nitrification rate. The inhibition produced by mixtures of ammonium chloride and sorbitol, each mixture generating an osmotic pressure of 5 atm. in the assay, was also investigated. The results suggest that inhibition by Cl-ion is disproportionate to its contribution to the osmotic pressure of the soil solution. The recovery of the nitrification rate, following exposure to high osmotic pressure solutions, was also investigated. It was found that the recovery of the nitrification rate was only partial, with the extent of the recovery diminishing as the severity of the initial osmotic stress applied increased. These results suggest that both reversible and irreversible mechanisms are involved in the inhibition of nitrification.
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  • 10
    ISSN: 1573-5036
    Keywords: Ammonium oxidation ; Clusters ; Modelling ; Nitrification ; pH effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The time course of nitrate production is different in different soils; in some soils, nitrate is produced at a constant rate while in others, this rate increases with time, often exponentially. Mechanistic models, based on the Monod equations, cannot account for a constant rate of nitrification. All such mechanistic models make the implicit assumption that the nitrifying organisms are distributed uniformly as single cells throughout the soil volume, while in reality, the cells might be expected to occur in small clusters formed by repeated cell division. This paper examines the effects of allowing the ammonium oxidising cells to occur in evenly distributed clusters of cells of equal volume. One effect of clustering would be the lowering of soil pH around the cluster, caused by differences in the rates of acid production and diffusion. The effects of this pH depression were examined using a mathematical model. In general, it was found that the effect of clustering was to reduce the rate of ammonium oxidation. In extreme cases, in which the fraction of the soil volume occupied by the cells was assumed to be small, the model predicted a constant rate of ammonium oxidation with time in contrast to the increasing rate with time predicted by a model based on a uniform single cell distribution. The clustering model was therefore capable of reproducing the different time courses of ammonium oxidation reported in the literature. The differences between the time courses of ammonium oxidation predicted using the two different assumptions was affected by the initial pH of the soil. This observation suggested a possible experimental test of the clustering hypothesis.
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