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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 28 (1990), S. 399-413 
    ISSN: 1573-4927
    Keywords: chimpanzee ; glycophorins ; glycophorin genes ; restriction fragment length polymorphism ; MN blood groups ; chimpanzee V-A-B-D blood groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Using human α glycophorin cDNA probe and six restriction enzymes, we examined the homologues of human glycophorin genes in genomic DNA of 11 unrelated chimpanzees. We show that, in contrast to the human, the chimpanzee exhibits an unusual array of nonrandomly distributed restriction fragment length polymorphisms (RFLP). No clear correlation was found between the RFLP and the V-A-B-D blood-group phenotypes of the subjects, with one possible exception. However, pairs of allelic RFLP occurring at a relatively high frequency were identified. In addition, the homology of chimpanzee glycophorin genes to the human genes was examined using as probes synthetic oligonucleotides specifying distinct regions of human glycophorin genes. We show that the glycophorin gene family in the chimpanzee consists of at least three members that are homologous to the human α, δ, and E genes (glycophorins A, B, and E) and may share a similar gross structure and overall organization.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 28 (1990), S. 399-413 
    ISSN: 1573-4927
    Keywords: chimpanzee ; glycophorins ; glycophorin genes ; restriction fragment length polymorphism ; MN blood groups ; chimpanzee V-A-B-D blood groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Using human α glycophorin cDNA probe and six restriction enzymes, we examined the homologues of human glycophorin genes in genomic DNA of 11 unrelated chimpanzees. We show that, in contrast to the human, the chimpanzee exhibits an unusual array of nonrandomly distributed restriction fragment length polymorphisms (RFLP). No clear correlation was found between the RFLP and the V-A-B-D blood-group phenotypes of the subjects, with one possible exception. However, pairs of allelic RFLP occurring at a relatively high frequency were identified. In addition, the homology of chimpanzee glycophorin genes to the human genes was examined using as probes synthetic oligonucleotides specifying distinct regions of human glycophorin genes. We show that the glycophorin gene family in the chimpanzee consists of at least three members that are homologous to the human α, δ, and E genes (glycophorins A, B, and E) and may share a similar gross structure and overall organization.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Key words Protein kinase ; Germination ; Fungal pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Colletotrichum trifolii is a fungal pathogen which is responsible for anthracnose disease of alfalfa. To initiate research on molecular communication in this fungus, a kinase-encoding gene (TB3) and the corresponding cDNA were cloned and sequenced. The deduced amino acid sequence of TB3 closely resembles that of a Neurospora crassa serine/threonine protein kinase, COT1, required for hyphal elongation and branching. The C-terminal catalytic domains of TB3 and COT1 are highly conserved but the N-terminal regions are divergent, particularly in the homopolymeric glutamine repeats of TB3. Northern analysis indicated that TB3 expression was highest 1 h after inducing conidial germination and 1 h before germ tubes were first observed. Expression of TB3 transcripts returned to constitutive levels by 4 h after induction of germination. TB3 complemented the cot-1 mutant of Neurospora crassa, demonstrating the functional conservation of this kinase between a pathogenic and a saprophytic fungus.
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  • 4
    ISSN: 1617-4623
    Keywords: Protein kinase ; Germination ; Fungal pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Colletotrichum trifolii is a fungal pathogen which is responsible for anthracnose disease of alfalfa. To initiate research on molecular communication in this fungus, a kinase-encoding gene (TB3) and the corresponding cDNA were cloned and sequenced. The deduced amino acid sequence ofTB3 closely resembles that of aNeurospora crassa serine/threonine protein kinase, COT1, required for hyphal elongation and branching. The C-terminal catalytic domains of TB3 and COT1 are highly conserved but the N-terminal regions are divergent, particularly in the homopolymeric glutamine repeats of TB3. Northern analysis indicated thatTB3 expression was highest 1 h after inducing conidial germination and 1 h before germ tubes were first observed. Expression of TB3 transcripts returned to constitutive levels by 4 h after induction of germination.TB3 complemented thecot-1 mutant ofNeurospora crassa, demonstrating the functional conservation of this kinase between a pathogenic and a saprophytic fungus.
    Type of Medium: Electronic Resource
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